2003
Study of the gene expression in malignant diseases by cDNA microarray technology
FALTÝSKOVÁ, Eva, Pavla GAJDUSKOVA a Stanislav KOZUBEKZákladní údaje
Originální název
Study of the gene expression in malignant diseases by cDNA microarray technology
Název česky
Studium genové exprese maligních onemocnění pomocí cDNA mikročipů
Autoři
FALTÝSKOVÁ, Eva (203 Česká republika), Pavla GAJDUSKOVA (203 Česká republika) a Stanislav KOZUBEK (203 Česká republika, garant)
Vydání
Brno, Biophysics of the Genome, od s. 25-29, 5 s. 2003
Nakladatel
Masaryk University
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
30200 3.2 Clinical medicine
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14330/03:00008566
Organizační jednotka
Fakulta informatiky
ISBN
80-210-3226-X
Klíčová slova anglicky
human genome structure
Štítky
Změněno: 4. 5. 2005 08:43, Ing. Libor Holuša, CSc.
V originále
At the present time, a number of various diseases increases and thereby also increases the necessity of new therapeutic methods and new approaches to research. In last decade, many new technologies have been evolving, among others the technology called cDNA microarrays. This technology enables the detection of global gene-expression profiles, the identification of new molecular cancer markers. It makes possible the comparison of gene-expression profiles of various cancer tumors and cell lines under different environmental stress conditions. cDNA microarrays consist of hundreds or thousands spots with different cDNA sequences (resp. oligonucleotide sequence strands) organized in grids and they can contain more than 20,000 specific sequences on a single slide. This technology is based on process of hybridization between immobilized cDNAs (oligonucleotides sequences) and reverse-transcribed cDNA from sample of total RNA. We used cDNA microarray technology to study gene expression profiles of colon cancer tissue derived from patients with colorectal carcinoma. Hereditary forms of colorectal carcinoma are often associated with familial polyposis syndromes (FAP) or hereditary nonpolyposis colorectal cancer (HNPCC). The mutations responsible for these syndromes are the mutations of APC gene (FAP) and DNA mismatch repair genes e.g. hMSH2, hMLH1 (HNPCC). Both pathways can be discerned in sporadic cases, but also many remaining genes can be deregulated. RNA, acquired from patients, was reverse-transcribed in the presence of fluorescent-labeled dCTP and was hybridized on the glass cDNA microarray. We identified genes, which were up- or down-regulated in tumor samples. In the next research we will study the topological organization of these genes in the nucleus by FISH technique.
Česky
Studium genové exprese maligních onemocnění pomocí cDNA mikročipů
Návaznosti
| MSM 143300002, záměr |
| ||
| NC6987, projekt VaV |
|