Analysis of DNA methylation status of cell nuclei using
high-resolution

D 2003

Analysis of DNA methylation status of cell nuclei using high-resolution

SKALNÍKOVÁ, Magdalena, Eva BÁRTOVÁ, Pavla GAJDUŠKOVÁ, Stanislav KOZUBEK, Michal KOZUBEK et. al.

Základní údaje

Originální název

Analysis of DNA methylation status of cell nuclei using high-resolution

Autoři

SKALNÍKOVÁ, Magdalena (203 Česká republika), Eva BÁRTOVÁ (203 Česká republika), Pavla GAJDUŠKOVÁ (203 Česká republika), Stanislav KOZUBEK (203 Česká republika, garant) a Michal KOZUBEK (203 Česká republika)

Vydání

Brno, Biophysics of the Genome, od s. 59-61, 3 s. 2003

Nakladatel

Masaryk University

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14330/03:00009009

Organizační jednotka

Fakulta informatiky

ISBN

80-210-3226-X

Klíčová slova anglicky

chromatin structure

Štítky

chromatin structure

Příznaky

Mezinárodní význam

Změněno: 29. 10. 2010 14:39, prof. RNDr. Michal Kozubek, Ph.D.

Anotace

V originále

Epigenetic silencing in eukaryotes is associated with the formation of a transcriptional repressive higher order chromatin structure which is also characterized by methylation of DNA at cytosine nucleotides. DNA methylation in eukaryotes involves of a methyl group to the carbon -5 position of the cytosine ring (5-mC). This reaction is catalysed by DNA methyltransferase in the context of the sequence 5'-GC-3', which is also referred to as a CpG dinucleotide. Differential methylation of CpG islands has been inversely correlated with gene activity: transcriptionally active genes were found to be hypomethylated, whereas transcriptionally silent genes were hypermethylated (Bird, A. 1992, Razin A. et al., 1991, Singal R., 1999). In our experiments in situ hybridisation technique and high-resolution cytometry were used to determine selected metaphase chromosomes HSA 1, 10, 18, 19 and 20. Sequential 5-methylcytosin (5-mC)-rich regions were visualised on metaphase chromosomes 1,10, 18, 19, and 20 using immunofluorescent labelling. The aim of this work was to study the methylation status at the chromosomal level in different cell types.

Návaznosti

IAB5004102, projekt VaV

Název: Jaderná topologie některých protoonkogenů u lidských neutrofilních granulocytů a leukemických buněk

Investor: Akademie věd ČR, Jaderná topologie některých protoonkogenů u lidských neutrofilních granulocytů a leukemických buněk

MSM 143300002, záměr

Název: Využití počítačové analýzy obrazu v optické mikroskopii

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Využití počítačové analýzy obrazu v optické mikroskopii

Citovat

SKALNÍKOVÁ, Magdalena, Eva BÁRTOVÁ, Pavla GAJDUŠKOVÁ, Stanislav KOZUBEK a Michal KOZUBEK. Analysis of DNA methylation status of cell nuclei using high-resolution. In Biophysics of the Genome. Brno: Masaryk University, 2003, s. 59-61. ISBN 80-210-3226-X.

@inproceedings{489662,
   author = {Skalníková, Magdalena and Bártová, Eva and Gajdušková, Pavla and Kozubek, Stanislav and Kozubek, Michal},
   address = {Brno},
   booktitle = {Biophysics of the Genome},
   keywords = {chromatin structure},
   language = {eng},
   location = {Brno},
   isbn = {80-210-3226-X},
   pages = {59-61},
   publisher = {Masaryk University},
   title = {Analysis of DNA methylation status of cell nuclei using high-resolution},
   year = {2003}
}

TY  - JOUR
ID  - 489662
AU  - Skalníková, Magdalena - Bártová, Eva - Gajdušková, Pavla - Kozubek, Stanislav - Kozubek, Michal
PY  - 2003
TI  - Analysis of DNA methylation status of cell nuclei using high-resolution
PB  - Masaryk University
CY  - Brno
SN  - 802103226X
KW  - chromatin structure
N2  - Epigenetic silencing in eukaryotes is associated with the formation of a transcriptional repressive higher order chromatin structure which is also characterized by methylation of DNA at cytosine nucleotides. DNA methylation in eukaryotes involves of a methyl group to the carbon -5 position of the cytosine ring (5-mC). This reaction is catalysed by DNA methyltransferase in the context of the sequence 5'-GC-3', which is also referred to as a CpG dinucleotide. Differential methylation of CpG islands has been inversely correlated with gene activity: transcriptionally active genes were found to be hypomethylated, whereas transcriptionally silent genes were hypermethylated (Bird, A. 1992, Razin A. et al., 1991, Singal R., 1999). In our experiments in situ hybridisation technique and high-resolution cytometry were used to determine selected metaphase chromosomes HSA 1, 10, 18, 19 and 20. Sequential 5-methylcytosin (5-mC)-rich regions were visualised on metaphase chromosomes 1,10, 18, 19, and 20 using immunofluorescent labelling. The aim of this work was to study the methylation status at the chromosomal level in different cell types.
ER  -

SKALNÍKOVÁ, Magdalena, Eva BÁRTOVÁ, Pavla GAJDUŠKOVÁ, Stanislav KOZUBEK a Michal KOZUBEK. Analysis of DNA methylation status of cell nuclei using high-resolution. In \textit{Biophysics of the Genome}. Brno: Masaryk University, 2003, s.~59-61. ISBN~80-210-3226-X.

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