The absence of Arabidopsis-type telomeres in Cestrum and
closely related genera Vestia and Sessea (Solanaceae): first
evidence from eudicots

J 2003

The absence of Arabidopsis-type telomeres in Cestrum and closely related genera Vestia and Sessea (Solanaceae): first evidence from eudicots

SÝKOROVÁ, Eva, Kar Yoong LIM, Mark W. CHASE, Sandra KNAPP, Ilia Judith LEITCH et. al.

Basic information

Original name

The absence of Arabidopsis-type telomeres in Cestrum and closely related genera Vestia and Sessea (Solanaceae): first evidence from eudicots

Authors

SÝKOROVÁ, Eva (203 Czech Republic), Kar Yoong LIM (826 United Kingdom of Great Britain and Northern Ireland), Mark W. CHASE (826 United Kingdom of Great Britain and Northern Ireland), Sandra KNAPP (826 United Kingdom of Great Britain and Northern Ireland), Ilia Judith LEITCH (826 United Kingdom of Great Britain and Northern Ireland), Andrew Rowland LEITCH (826 United Kingdom of Great Britain and Northern Ireland) and Jiří FAJKUS (203 Czech Republic, guarantor)

Edition

Plant Journal, Oxford, Blackwell Publishing Ltd, 2003, 0960-7412

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 5.914

RIV identification code

RIV/00216224:14310/03:00008258

Organization unit

Faculty of Science

UT WoS

000182455200003

Keywords in English

absence of TTTAGGG telomere; dicot; chromosome; FISH

Abstract

V originále

Using slot-blot and fluorescent in situ hybridization (FISH), we found no evidence for the presence of the Arabidopsis-type telomeric sequence (TTTAGGG)n at the chromosome termini in any of the Cestrum species we investigated. Probing for the human-type telomere (TTAGGG)n also revealed no signal. However, polymerase chain reaction experiments indicated that there are short lengths of the sequence TTTAGGG dispersed in the genome but that these sequences are almost certainly too short to act as functional telomeres even if they were at the chromosome termini. An analysis of related genera Vestia and Sessea indicates that they too lack the Arabidopsis-type telomere, and the sequences were lost in the common ancestor of these genera. We found that the Cestrum species investigated had particularly large mean chromosome sizes. We discuss whether this is a consequence of alternative telomere end maintenance systems.

Links

GA204/02/0027, research and development project

Name: Molekulární analýza struktury a funkce typických a alternativních telomer u rostlin

MSM 143100008, plan (intention)

Name: Genomy a jejich funkce

Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions

Citovat

SÝKOROVÁ, Eva, Kar Yoong LIM, Mark W. CHASE, Sandra KNAPP, Ilia Judith LEITCH, Andrew Rowland LEITCH and Jiří FAJKUS. The absence of Arabidopsis-type telomeres in Cestrum and closely related genera Vestia and Sessea (Solanaceae): first evidence from eudicots. Plant Journal. Oxford: Blackwell Publishing Ltd, 2003, Neuveden, No 34, p. 283-291. ISSN 0960-7412.

@article{489747,
   author = {Sýkorová, Eva and Lim, Kar Yoong and Chase, Mark W. and Knapp, Sandra and Leitch, Ilia Judith and Leitch, Andrew Rowland and Fajkus, Jiří},
   article_location = {Oxford},
   article_number = {34},
   keywords = {absence of TTTAGGG telomere; dicot; chromosome; FISH},
   language = {eng},
   issn = {0960-7412},
   journal = {Plant Journal},
   title = {The absence of Arabidopsis-type telomeres in Cestrum and closely related genera Vestia and Sessea (Solanaceae): first evidence from eudicots},
   volume = {Neuveden},
   year = {2003}
}

TY  - JOUR
ID  - 489747
AU  - Sýkorová, Eva - Lim, Kar Yoong - Chase, Mark W. - Knapp, Sandra - Leitch, Ilia Judith - Leitch, Andrew Rowland - Fajkus, Jiří
PY  - 2003
TI  - The absence of Arabidopsis-type telomeres in Cestrum and closely related genera Vestia and Sessea (Solanaceae): first evidence from eudicots
JF  - Plant Journal
VL  - Neuveden
IS  - 34
SP  - 283
EP  - 283
PB  - Blackwell Publishing Ltd
SN  - 09607412
KW  - absence of TTTAGGG telomere
KW  - dicot
KW  - chromosome
KW  - FISH
N2  - Using slot-blot and fluorescent in situ hybridization (FISH), we found no evidence for the presence of the Arabidopsis-type telomeric sequence (TTTAGGG)n at the chromosome termini in any of the Cestrum species we investigated. Probing for the human-type telomere (TTAGGG)n also revealed no signal. However, polymerase chain reaction experiments indicated that there are short lengths of the sequence TTTAGGG dispersed in the genome but that these sequences are almost certainly too short to act as functional telomeres even if they were at the chromosome termini. An analysis of related genera Vestia and Sessea indicates that they too lack the Arabidopsis-type telomere, and the sequences were lost in the common ancestor of these genera. We found that the Cestrum species investigated had particularly large mean chromosome sizes. We discuss whether this is a consequence of alternative telomere end maintenance systems.
ER  -

SÝKOROVÁ, Eva, Kar Yoong LIM, Mark W. CHASE, Sandra KNAPP, Ilia Judith LEITCH, Andrew Rowland LEITCH and Jiří FAJKUS. The absence of Arabidopsis-type telomeres in Cestrum and closely related genera Vestia and Sessea (Solanaceae): first evidence from eudicots. \textit{Plant Journal}. Oxford: Blackwell Publishing Ltd, 2003, Neuveden, No~34, p.~283-291. ISSN~0960-7412.

Detailed Information on Publication Record