Detailed Information on Publication Record
2003
Migration Stimulating Factor (MSF/FN70): a genetically truncated fibronectin expressed by carcinoma and tumor-associated stromal cells
SCHOR, S.L., I.R. ELLIS, S.J. JONES, K. SENEVIRATNE, R. BAILLIE et. al.Basic information
Original name
Migration Stimulating Factor (MSF/FN70): a genetically truncated fibronectin expressed by carcinoma and tumor-associated stromal cells
Name in Czech
Migration Stimulating Factor (MSF/FN70): geneticky zkraceny fibronektin exprimovany stromalnimi bunkami tumoru
Authors
SCHOR, S.L. (826 United Kingdom of Great Britain and Northern Ireland), I.R. ELLIS (826 United Kingdom of Great Britain and Northern Ireland), S.J. JONES (826 United Kingdom of Great Britain and Northern Ireland), K. SENEVIRATNE (826 United Kingdom of Great Britain and Northern Ireland), R. BAILLIE (826 United Kingdom of Great Britain and Northern Ireland), J. CLAUSEN (826 United Kingdom of Great Britain and Northern Ireland), K. MOTEGI (392 Japan), Bořivoj VOJTĚŠEK (203 Czech Republic, guarantor), Kateřina KAŇKOVÁ (203 Czech Republic), E. FURRIE (826 United Kingdom of Great Britain and Northern Ireland), M.J. SALES (826 United Kingdom of Great Britain and Northern Ireland), A.M. SCHOR (826 United Kingdom of Great Britain and Northern Ireland) and R.A. KAY (826 United Kingdom of Great Britain and Northern Ireland)
Edition
Cancer Research, USA, American Association for Cancer Research, 2003, 0008-5472
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 8.649
RIV identification code
RIV/00216224:14110/03:00009878
Organization unit
Faculty of Medicine
UT WoS
000187580300037
Keywords in English
MSF; fibronectin; cancer pathogenesis; cell motility; onco-fetal protein
Změněno: 23/6/2009 15:24, prof. MUDr. Kateřina Kaňková, Ph.D.
V originále
Migration-stimulating factor (MSF) is a 70-kDa motogenic protein previously reported to be expressed by fetal and cancer patient fibroblasts cultured in vitro and present in the serum of breast cancer patients. A 2.2-kb full-length MSF cDNA has been cloned and shown to be a truncated isoform of fibronectin generated from its primary gene transcript by a hitherto unrecognized intron read-through mechanism. MSF cDNA is identical to the 5' end of fibronectin cDNA, up to and including exon III-1a, and terminates in a novel 195-nucleotide 3' sequence. This MSF unique sequence is derived from the intron immediately downstream of exon III-1a in the fibronectin gene and is not found in any previously identified "full-length" fibronectin cDNA. MSF mRNA is 1000-fold less abundant than full-length fibronectin message in fetal fibroblasts and exhibits rapid biphasic decay kinetics previously associated with oncogenes and stress response molecules. MSF recombinant protein exhibits a potent and substratum-dependent motogenic activity, with half-maximal response manifest at 0.1-1.0 pg/ml. This activity is (a) mediated by the IGD amino acid motif; and (b) not expressed by (i.e., cryptic within) full-length fibronectin. In situ hybridization and immunohistochemistry confirm that MSF is expressed by tumor-associated fibroblasts and additionally indicate that it is also expressed by carcinoma cells and tumor-associated vascular endothelial cells. MSF, as a consequence of its potent bioactivities and expression by both stromal and carcinoma cell populations, is well placed to function as an epigenetic effector promoting cancer development.
In Czech
Nove popsany protein MSF se uplatnuje jako epigeneticky faktor lokalne ovlivnujici progresi tumoru.
Links
LZ1K03019, research and development project |
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