Migration Stimulating Factor (MSF/FN70): a genetically
truncated fibronectin expressed by carcinoma and
tumor-associated stromal cells

J 2003

Migration Stimulating Factor (MSF/FN70): a genetically truncated fibronectin expressed by carcinoma and tumor-associated stromal cells

SCHOR, S.L., I.R. ELLIS, S.J. JONES, K. SENEVIRATNE, R. BAILLIE et. al.

Basic information

Original name

Migration Stimulating Factor (MSF/FN70): a genetically truncated fibronectin expressed by carcinoma and tumor-associated stromal cells

Name in Czech

Migration Stimulating Factor (MSF/FN70): geneticky zkraceny fibronektin exprimovany stromalnimi bunkami tumoru

Authors

SCHOR, S.L. (826 United Kingdom of Great Britain and Northern Ireland), I.R. ELLIS (826 United Kingdom of Great Britain and Northern Ireland), S.J. JONES (826 United Kingdom of Great Britain and Northern Ireland), K. SENEVIRATNE (826 United Kingdom of Great Britain and Northern Ireland), R. BAILLIE (826 United Kingdom of Great Britain and Northern Ireland), J. CLAUSEN (826 United Kingdom of Great Britain and Northern Ireland), K. MOTEGI (392 Japan), Bořivoj VOJTĚŠEK (203 Czech Republic, guarantor), Kateřina KAŇKOVÁ (203 Czech Republic), E. FURRIE (826 United Kingdom of Great Britain and Northern Ireland), M.J. SALES (826 United Kingdom of Great Britain and Northern Ireland), A.M. SCHOR (826 United Kingdom of Great Britain and Northern Ireland) and R.A. KAY (826 United Kingdom of Great Britain and Northern Ireland)

Edition

Cancer Research, USA, American Association for Cancer Research, 2003, 0008-5472

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 8.649

RIV identification code

RIV/00216224:14110/03:00009878

Organization unit

Faculty of Medicine

UT WoS

000187580300037

Keywords in English

MSF; fibronectin; cancer pathogenesis; cell motility; onco-fetal protein

Abstract

ORIG CZ

V originále

Migration-stimulating factor (MSF) is a 70-kDa motogenic protein previously reported to be expressed by fetal and cancer patient fibroblasts cultured in vitro and present in the serum of breast cancer patients. A 2.2-kb full-length MSF cDNA has been cloned and shown to be a truncated isoform of fibronectin generated from its primary gene transcript by a hitherto unrecognized intron read-through mechanism. MSF cDNA is identical to the 5' end of fibronectin cDNA, up to and including exon III-1a, and terminates in a novel 195-nucleotide 3' sequence. This MSF unique sequence is derived from the intron immediately downstream of exon III-1a in the fibronectin gene and is not found in any previously identified "full-length" fibronectin cDNA. MSF mRNA is 1000-fold less abundant than full-length fibronectin message in fetal fibroblasts and exhibits rapid biphasic decay kinetics previously associated with oncogenes and stress response molecules. MSF recombinant protein exhibits a potent and substratum-dependent motogenic activity, with half-maximal response manifest at 0.1-1.0 pg/ml. This activity is (a) mediated by the IGD amino acid motif; and (b) not expressed by (i.e., cryptic within) full-length fibronectin. In situ hybridization and immunohistochemistry confirm that MSF is expressed by tumor-associated fibroblasts and additionally indicate that it is also expressed by carcinoma cells and tumor-associated vascular endothelial cells. MSF, as a consequence of its potent bioactivities and expression by both stromal and carcinoma cell populations, is well placed to function as an epigenetic effector promoting cancer development.

In Czech

Nove popsany protein MSF se uplatnuje jako epigeneticky faktor lokalne ovlivnujici progresi tumoru.

Links

LZ1K03019, research and development project

Name: Zlepšení hojení ran u pacientů s diabetem: preklinické ověření role proteinu MSF

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

SCHOR, S.L., I.R. ELLIS, S.J. JONES, K. SENEVIRATNE, R. BAILLIE, J. CLAUSEN, K. MOTEGI, Bořivoj VOJTĚŠEK, Kateřina KAŇKOVÁ, E. FURRIE, M.J. SALES, A.M. SCHOR and R.A. KAY. Migration Stimulating Factor (MSF/FN70): a genetically truncated fibronectin expressed by carcinoma and tumor-associated stromal cells. Cancer Research. USA: American Association for Cancer Research, 2003, vol. 63, No 24, p. 8827-8836. ISSN 0008-5472.

@article{490094,
   author = {Schor, S.L. and Ellis, I.R. and Jones, S.J. and Seneviratne, K. and Baillie, R. and Clausen, J. and Motegi, K. and Vojtěšek, Bořivoj and Kaňková, Kateřina and Furrie, E. and Sales, M.J. and Schor, A.M. and Kay, R.A.},
   article_location = {USA},
   article_number = {24},
   keywords = {MSF; fibronectin; cancer pathogenesis; cell motility; onco-fetal protein},
   language = {eng},
   issn = {0008-5472},
   journal = {Cancer Research},
   title = {Migration Stimulating Factor (MSF/FN70): a genetically truncated fibronectin expressed by carcinoma and tumor-associated stromal cells},
   volume = {63},
   year = {2003}
}

TY  - JOUR
ID  - 490094
AU  - Schor, S.L. - Ellis, I.R. - Jones, S.J. - Seneviratne, K. - Baillie, R. - Clausen, J. - Motegi, K. - Vojtěšek, Bořivoj - Kaňková, Kateřina - Furrie, E. - Sales, M.J. - Schor, A.M. - Kay, R.A.
PY  - 2003
TI  - Migration Stimulating Factor (MSF/FN70): a genetically truncated fibronectin expressed by carcinoma and tumor-associated stromal cells
JF  - Cancer Research
VL  - 63
IS  - 24
SP  - 8827
EP  - 8827
PB  - American Association for Cancer Research
SN  - 00085472
KW  - MSF
KW  - fibronectin
KW  - cancer pathogenesis
KW  - cell motility
KW  - onco-fetal protein
N2  - Migration-stimulating factor (MSF) is a 70-kDa motogenic protein previously reported to be expressed by fetal and cancer patient fibroblasts cultured in vitro and present in the serum of breast cancer patients. A 2.2-kb full-length MSF cDNA has been cloned and shown to be a truncated isoform of fibronectin generated from its primary gene transcript by a hitherto unrecognized intron read-through mechanism. MSF cDNA is identical to the 5' end of fibronectin cDNA, up to and including exon III-1a, and terminates in a novel 195-nucleotide 3' sequence. This MSF unique sequence is derived from the intron immediately downstream of exon III-1a in the fibronectin gene and is not found in any previously identified "full-length" fibronectin cDNA. MSF mRNA is 1000-fold less abundant than full-length fibronectin message in fetal fibroblasts and exhibits rapid biphasic decay kinetics previously associated with oncogenes and stress response molecules. MSF recombinant protein exhibits a potent and substratum-dependent motogenic activity, with half-maximal response manifest at 0.1-1.0 pg/ml. This activity is (a) mediated by the IGD amino acid motif; and (b) not expressed by (i.e., cryptic within) full-length fibronectin. In situ hybridization and immunohistochemistry confirm that MSF is expressed by tumor-associated fibroblasts and additionally indicate that it is also expressed by carcinoma cells and tumor-associated vascular endothelial cells. MSF, as a consequence of its potent bioactivities and expression by both stromal and carcinoma cell populations, is well placed to function as an epigenetic effector promoting cancer development.
ER  -

SCHOR, S.L., I.R. ELLIS, S.J. JONES, K. SENEVIRATNE, R. BAILLIE, J. CLAUSEN, K. MOTEGI, Bořivoj VOJTĚŠEK, Kateřina KAŇKOVÁ, E. FURRIE, M.J. SALES, A.M. SCHOR and R.A. KAY. Migration Stimulating Factor (MSF/FN70): a genetically truncated fibronectin expressed by carcinoma and tumor-associated stromal cells. \textit{Cancer Research}. USA: American Association for Cancer Research, 2003, vol.~63, No~24, p.~8827-8836. ISSN~0008-5472.

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