Structural basis of calcium and galactose recognition by the
lectin PA IL of Pseudomonas aeruginosa

J 2003

Structural basis of calcium and galactose recognition by the lectin PA IL of Pseudomonas aeruginosa

CIOCCI, Gianluca, Edward MITCHELL, Catherine GAUIER, Michaela WIMMEROVÁ, Dvora SUDAKEVITZ et. al.

Základní údaje

Originální název

Structural basis of calcium and galactose recognition by the lectin PA IL of Pseudomonas aeruginosa

Autoři

CIOCCI, Gianluca (380 Itálie), Edward MITCHELL (826 Velká Británie a Severní Irsko), Catherine GAUIER (250 Francie), Michaela WIMMEROVÁ (203 Česká republika, garant), Dvora SUDAKEVITZ (376 Izrael), Serge PÉREZ (250 Francie), Nechama GILBOA-GARBER (376 Izrael) a Anne IMBERTY (250 Francie)

Vydání

FEBS Letters, Amsterdam, Elsevier, 2003, 0014-5793

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.609

Kód RIV

RIV/00216224:14310/03:00009169

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

pseudomonas aeruginosa; cystic fibrosis; lectin; crystal structure

Štítky

Crystal Structure, cystic fibrosis, lectin, Pseudomonas aeruginosa

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 1. 2007 15:32, prof. RNDr. Michaela Wimmerová, Ph.D.

Anotace

V originále

The structure of the tetrameric Pseudomonas aeruginosa lectin I (PA-IL) in complex with galactose and calcium was determined at 1.6 Å esolution, and the native protein was solved at 2.4 Å resolution. Each monomer adopts a beta-sandwich fold with ligand binding site at the apex. All galactose hydroxyl groups, except O1, are involved in a hydrogen bond network with the protein and O3 and O4 also participate in the coordination of the calcium ion. The stereochemistry of calcium galactose binding is reminiscent to that observed in some animal C-type lectins. The structure of the complex provides a framework for future design of anti-bacterial compounds.

Návaznosti

ME 675, projekt VaV

Název: Strukturně- funkční studie lektinů bakteriálních patogenů

MSM 143100005, záměr

Název: Strukturně-funkční vztahy biomolekul a jejich role v metabolismu

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Strukturně-funkční vztahy biomolekul a jejich role v metabolismu

Citovat

CIOCCI, Gianluca, Edward MITCHELL, Catherine GAUIER, Michaela WIMMEROVÁ, Dvora SUDAKEVITZ, Serge PÉREZ, Nechama GILBOA-GARBER a Anne IMBERTY. Structural basis of calcium and galactose recognition by the lectin PA IL of Pseudomonas aeruginosa. FEBS Letters. Amsterdam: Elsevier, 2003, roč. 552, č. 2, s. 297-301. ISSN 0014-5793.

@article{490424,
   author = {Ciocci, Gianluca and Mitchell, Edward and Gauier, Catherine and Wimmerová, Michaela and Sudakevitz, Dvora and Pérez, Serge and GilboaandGarber, Nechama and Imberty, Anne},
   article_location = {Amsterdam},
   article_number = {2},
   keywords = {pseudomonas aeruginosa; cystic fibrosis; lectin; crystal structure},
   language = {eng},
   issn = {0014-5793},
   journal = {FEBS Letters},
   title = {Structural basis of calcium and galactose recognition by the lectin PA IL of Pseudomonas aeruginosa},
   volume = {552},
   year = {2003}
}

TY  - JOUR
ID  - 490424
AU  - Ciocci, Gianluca - Mitchell, Edward - Gauier, Catherine - Wimmerová, Michaela - Sudakevitz, Dvora - Pérez, Serge - Gilboa-Garber, Nechama - Imberty, Anne
PY  - 2003
TI  - Structural basis of calcium and galactose recognition by the lectin PA IL of Pseudomonas aeruginosa
JF  - FEBS Letters
VL  - 552
IS  - 2
SP  - 297-301
EP  - 297-301
PB  - Elsevier
SN  - 00145793
KW  - pseudomonas aeruginosa
KW  - cystic fibrosis
KW  - lectin
KW  - crystal structure
N2  - The structure of the tetrameric Pseudomonas aeruginosa lectin I (PA-IL) in complex with galactose and calcium was determined at 1.6 Å esolution, and the native protein was solved at 2.4 Å resolution. Each monomer adopts a beta-sandwich fold with ligand binding site at the apex. All galactose hydroxyl groups, except O1, are involved in a hydrogen bond network with the protein and O3 and O4 also participate in the coordination of the calcium ion. The stereochemistry of calcium galactose binding is reminiscent to that observed in some animal C-type lectins. The structure of the complex provides a framework for future design of anti-bacterial compounds.
ER  -

CIOCCI, Gianluca, Edward MITCHELL, Catherine GAUIER, Michaela WIMMEROVÁ, Dvora SUDAKEVITZ, Serge PÉREZ, Nechama GILBOA-GARBER a Anne IMBERTY. Structural basis of calcium and galactose recognition by the lectin PA IL of Pseudomonas aeruginosa. \textit{FEBS Letters}. Amsterdam: Elsevier, 2003, roč.~552, č.~2, s.~297-301. ISSN~0014-5793.

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