DUBOVÝ, Petr, Radim JANČÁLEK and Ilona KLUSÁKOVÁ. Changes of immunofluorescence staining for ECM molecules in the satellite cells of rat dorsal root ganglia following a chronic constriction injury of the peripheral nerve. Glia Suppl. USA: Wiley-Liss, Inc., 2003, vol. 2, No 1, p. 36-37. ISSN 0894-1491.
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Basic information
Original name Changes of immunofluorescence staining for ECM molecules in the satellite cells of rat dorsal root ganglia following a chronic constriction injury of the peripheral nerve
Authors DUBOVÝ, Petr (203 Czech Republic, guarantor), Radim JANČÁLEK (203 Czech Republic) and Ilona KLUSÁKOVÁ (203 Czech Republic).
Edition Glia Suppl, USA, Wiley-Liss, Inc. 2003, 0894-1491.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30000 3. Medical and Health Sciences
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 4.677
RIV identification code RIV/00216224:14110/03:00008386
Organization unit Faculty of Medicine
UT WoS 000184938300154
Keywords in English laminin; chondroitin sulfate; tenascin; fibronectin; endoneurium
Tags chondroitin sulfate, endoneurium, fibronectin, laminin, tenascin
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Petr Dubový, CSc., učo 698. Changed: 26/6/2009 10:49.
Abstract
We have investigated immunostaining for some ECM molecules ( chondroitin sulfate, tenascin, thrombospondin, fibronectin) in the DRG satellite cells following CCI of the peripheral nerve. The experimental CCI was applied ipsilaterally on the spinal nerve and the sciatic nerve of Wistar rats (12 females, 280g). Both ipsilateral and contralateral DRG (L4-L5) were removed 1, 2, 3, 5 weeks after the operation. The immunofluorescence staining for ECM molecules was compared in the sections through ipsilateral and contralateral DRG by an image analysis system. The satellite cell sheaths of ipsilateral DRG (L4-L5) displayed a higher brightness of immunofluorescence staining for investigated ECM molecules in comparison to the contralateral ones. The changes were more noticeable after CCI applied near to DRG (4mm) than in longer distance (40mm). An increased immunostaining for the ECM molecules in the satellite cells following CCI of peripheral nerve suggested that the changes may be implicated in growth stimuli for sympathetic sprouts and formation of sympathetic baskets.
Abstract (in Czech)
Molekuly ECM (CSPG, tenascin, thrombospondin, fibronectin) byly imunohistochemicky detekovány ve spinálních gangliích po chronické konstrikci nervu.
Links
GA309/00/0407, research and development projectName: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů
Investor: Czech Science Foundation, Cellular and extracellular environment for the axon regenaration following injury of the spinal roots and peripheral nerves
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