2003
Sensitivity of photosystem 2 of Antarctic lichens to high irradiance stress:Fluorometric study of fruticose (Usnea antarctica)and foliose (Umbilicaria decussata) species
BARTÁK, Miloš, Hana VRÁBLÍKOVÁ a Josef HÁJEKZákladní údaje
Originální název
Sensitivity of photosystem 2 of Antarctic lichens to high irradiance stress:Fluorometric study of fruticose (Usnea antarctica)and foliose (Umbilicaria decussata) species
Název anglicky
Sensitivity of photosystem 2 of Antarctic lichens to high irradiance stress:Fluorometric study of fruticose (Usnea antarctica)and foliose (Umbilicaria decussata) species
Autoři
BARTÁK, Miloš (203 Česká republika, garant), Hana VRÁBLÍKOVÁ (203 Česká republika) a Josef HÁJEK (203 Česká republika)
Vydání
Photosynthetica, Praha, Elsevier, 2003, 0300-3604
Další údaje
Jazyk
čeština
Typ výsledku
Článek v odborném periodiku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 0.661
Kód RIV
RIV/00216224:14310/03:00008618
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
chlorophyll fluorescence; low temperature; photoinhibition; photosynthesis; non-photochemical quenching; recovery.
Štítky
Změněno: 23. 4. 2004 10:12, prof. Ing. Miloš Barták, CSc.
V originále
Two lichen species collected in maritime Antarctica (King George Island) were exposed under laboratory conditions to excess irradiance to evaluate the response of photosystem 2 (PS2). The response was measured on fully hydrated lichen thalli at 5 oC by means of a modulated fluorometer using chlorophyll (Chl) fluorescence induction curve supplemented with analysis of quenching mechanisms. Chl fluorescence parameters [i.e. ratio of variable to maximum Chl fluorescence (FV/FM), quantum yield of PS2 photochemical reactions (?2), quenching coefficients] were evaluated before and several times after exposition to high irradiance in order to characterise the extent of photoinhibition, fast and slow phase of recovery. Strong irradiance (2 000 ľmol m-2 s-1) caused high degree of photoinhibition, particularly higher in fruticose (Usnea antarctica) than in foliose (Umbilicaria decussata) lichen species. Fast phase of recovery from photoinhibition, corresponding to regulatory mechanisms of PS2, was apparent in both species for FV/FM and ?2 within 40 min after photoinhibitory treatment. It was followed by a slow phase lasting several hours, corresponding to repair and re-synthesis processes. After photoinhibitory treatment, recovery of non-photochemical quenching (NPQ) was faster and more pronounced in U. decussata than in U. antarctica. Significant differences were found between the two species in the rate of recovery in fast- (qE) and slow-recovering (qT+I) component of NPQ.
Anglicky
Two lichen species collected in maritime Antarctica (King George Island) were exposed under laboratory conditions to excess irradiance to evaluate the response of photosystem 2 (PS2). The response was measured on fully hydrated lichen thalli at 5 oC by means of a modulated fluorometer using chlorophyll (Chl) fluorescence induction curve supplemented with analysis of quenching mechanisms. Chl fluorescence parameters [i.e. ratio of variable to maximum Chl fluorescence (FV/FM), quantum yield of PS2 photochemical reactions (?2), quenching coefficients] were evaluated before and several times after exposition to high irradiance in order to characterise the extent of photoinhibition, fast and slow phase of recovery. Strong irradiance (2 000 ľmol m-2 s-1) caused high degree of photoinhibition, particularly higher in fruticose (Usnea antarctica) than in foliose (Umbilicaria decussata) lichen species. Fast phase of recovery from photoinhibition, corresponding to regulatory mechanisms of PS2, was apparent in both species for FV/FM and ?2 within 40 min after photoinhibitory treatment. It was followed by a slow phase lasting several hours, corresponding to repair and re-synthesis processes. After photoinhibitory treatment, recovery of non-photochemical quenching (NPQ) was faster and more pronounced in U. decussata than in U. antarctica. Significant differences were found between the two species in the rate of recovery in fast- (qE) and slow-recovering (qT+I) component of NPQ.
Návaznosti
| GA522/03/0754, projekt VaV |
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| MSM 143100007, záměr |
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