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@article{493103,
author = {Rittich, Bohuslav and Španová, Alena and Falk, Martin and Beneš, Milan and Hrubý, Martin},
article_location = {Amsterdam},
article_number = {1-2},
keywords = {Cleavage; Lanthanide icomplexes; Plasmid DNA pBR322},
language = {eng},
issn = {1570-0232},
journal = {Journal of Chromatography B},
title = {Cleavage of double stranded plasmid DNA by lanthanide complexes},
volume = {800},
year = {2004}
}
TY - JOUR
ID - 493103
AU - Rittich, Bohuslav - Španová, Alena - Falk, Martin - Beneš, Milan - Hrubý, Martin
PY - 2004
TI - Cleavage of double stranded plasmid DNA by lanthanide complexes
JF - Journal of Chromatography B
VL - 800
IS - 1-2
SP - 169-173
EP - 169-173
PB - Elsevier
SN - 15700232
KW - Cleavage
KW - Lanthanide icomplexes
KW - Plasmid DNA pBR322
N2 - The use of free lanthanide ions and their complexes for plasmid DNA pBR322 and chromosomal DNA was studied. Plasmid pBR322 DNA was treated by lanthanide chlorides (Eu3+, La3+, Nd3+, Pr3+, Gd3+ ) in HEPES buffer (pH 7.0, 7.5 and 8.0) at different temperatures. The formation of linear and nicked plasmid forms was investigated depending on the reaction conditions. Heterogeneous lanthanide complexes of ethylenediamine tetraacetic acid (EDTA) immobilized on insoluble methacrylate support and iminodiacetic acid (IDA) immobilized on styrene support were used as catalysts plasmid for DNA pBR322 cleavage, too. The temperature of reaction mixture had substantial influence on cleavage rate. The precipitation of DNA occured during the measurement of interactions between chromosomal DNA and La3+ ions.
ER -
RITTICH, Bohuslav, Alena ŠPANOVÁ, Martin FALK, Milan BENEŠ a Martin HRUBÝ. Cleavage of double stranded plasmid DNA by lanthanide complexes. \textit{Journal of Chromatography B}. Amsterdam: Elsevier, 2004, roč.~800, 1-2, s.~169-173. ISSN~1570-0232.
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