Differential effects of transcription coactivators CBP and p300 on v-myb-transfromed monoblasts

NEMAJEROVÁ, Alice, Jan ŠMARDA, Jiřina ZATLOUKALOVÁ a Jana ŠMARDOVÁ. Differential effects of transcription coactivators CBP and p300 on v-myb-transfromed monoblasts. In Memory in Living Systems. Brno: Czechoslovak Biological Society, 2003, s. 66. ISBN 80-210-3264-2.

Základní údaje

• Originální název: Differential effects of transcription coactivators CBP and p300 on v-myb-transfromed monoblasts
• Název anglicky: Differential effects of transcription coactivators CBP and p300 on v-myb-transfromed monoblasts
• Autoři: NEMAJEROVÁ, Alice (203 Česká republika), Jan ŠMARDA (203 Česká republika), Jiřina ZATLOUKALOVÁ (203 Česká republika) a Jana ŠMARDOVÁ (203 Česká republika, garant).
• Vydání: Brno, Memory in Living Systems, s. 66-66, 2003.
• Nakladatel: Czechoslovak Biological Society

Další údaje

• Originální jazyk: čeština
• Typ výsledku: Stať ve sborníku
• Obor: Genetika a molekulární biologie
• Stát vydavatele: Česká republika
• Utajení: není předmětem státního či obchodního tajemství
• Kód RIV: RIV/00216224:14310/03:00009569
• Organizační jednotka: Přírodovědecká fakulta
• ISBN: 80-210-3264-2
• Klíčová slova anglicky: CBP; p300; Myb; histone acetylation
• Štítky: CBP, histone acetylation, Myb, p300

Anotace

The v-myb oncogene is efficiently expressed in BM2 monoblasts thus blocking their terminal differentiation steps. Histone acetylation plays an important role in regulation of transcription and is particularly relevant to regulation and pathology of hematopoiesis. Recently, we have shown that inhibitor of histone deacetylases trichostatin A (TSA) causes histone hyperacetylation and differentiation of BM2 cells into macrophage polycaryons. In this work we used another approach to stimulate histone acetylation in BM2 cells. We prepared two variants of BM2 cells ectopically expressing two histone acetyl transferases CBP and p300. The expression of CBP as well as p300 itself did not affect the phenotype of BM2 cells, but it enhanced their response to the differentiation inducers TPA and TSA. Although CBP and p300 are closely related proteins, many differences in their function have been described. We noted differences in kinetics of differentiation induced by CBP and p300 in BM2 cells.

Anotace anglicky

The v-myb oncogene is efficiently expressed in BM2 monoblasts thus blocking their terminal differentiation steps. Histone acetylation plays an important role in regulation of transcription and is particularly relevant to regulation and pathology of hematopoiesis. Recently, we have shown that inhibitor of histone deacetylases trichostatin A (TSA) causes histone hyperacetylation and differentiation of BM2 cells into macrophage polycaryons. In this work we used another approach to stimulate histone acetylation in BM2 cells. We prepared two variants of BM2 cells ectopically expressing two histone acetyl transferases CBP and p300. The expression of CBP as well as p300 itself did not affect the phenotype of BM2 cells, but it enhanced their response to the differentiation inducers TPA and TSA. Although CBP and p300 are closely related proteins, many differences in their function have been described. We noted differences in kinetics of differentiation induced by CBP and p300 in BM2 cells.

Návaznosti

• MSM 143100008, záměr: Název: Genomy a jejich funkce

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Genomy a jejich funkce