Differential effects of c-Fos and v-Fos proteins on v-myb-transformed monoblasts

ZAHRADNÍČKOVÁ, Eva, Sabina ŠEVČÍKOVÁ, Karel SOUČEK and Jan ŠMARDA. Differential effects of c-Fos and v-Fos proteins on v-myb-transformed monoblasts. In Memory in Living Systems. Brno: Czechoslovak Biological Society, 2003, p. 73. ISBN 80-210-3264-2.

Basic information

• Original name: Differential effects of c-Fos and v-Fos proteins on v-myb-transformed monoblasts
• Name (in English): Differential effects of c-Fos and v-Fos proteins on v-myb-transformed monoblasts
• Authors: ZAHRADNÍČKOVÁ, Eva (203 Czech Republic), Sabina ŠEVČÍKOVÁ (203 Czech Republic), Karel SOUČEK (203 Czech Republic) and Jan ŠMARDA (203 Czech Republic, guarantor).
• Edition: Brno, Memory in Living Systems, p. 73-73, 2003.
• Publisher: Czechoslovak Biological Society

Other information

• Original language: Czech
• Type of outcome: Proceedings paper
• Field of Study: Genetics and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14330/03:00008669
• Organization unit: Faculty of Informatics
• ISBN: 80-210-3264-2
• Keywords in English: Fos; Myb; proliferation; differentiation; apoptosis
• Tags: apoptosis, differentiation, Fos, Myb, proliferation

Abstract

c-Fos and v-Fos proteins as well as v-Jun and c-Jun belong to the proteins forming the AP-1 transcription factor. The AP-1 participates in regulation of proliferation, differentiation and programmed cell death in multiple cell types. In this study we explored the effects of c-Fos and v-Fos proteins on the line of v-myb transformed chicken monoblasts BM2. Both c-Fos and v-Fos proteins were ectopically expressed in BM2 cells. The v-Fos protein induced growth arrest in G0G1-phase of the BM2 cell cycle similarly as c-Jun protein. Interestingly, c-Fos leaved the cell cycle unaffected and rather induced programmed cell death. This effect of c-Fos protein was markedly enhanced in cells cultivated under serum-free conditions. In contrast to Jun proteins, no differentiation promoting effect of c-Fos and v-Fos protein was detected in BM2 cells. These results suggest differential roles of individual components of the AP-1 transcription factor in regulation of differentiation and programmed cell.

Abstract (in English)

c-Fos and v-Fos proteins as well as v-Jun and c-Jun belong to the proteins forming the AP-1 transcription factor. The AP-1 participates in regulation of proliferation, differentiation and programmed cell death in multiple cell types. In this study we explored the effects of c-Fos and v-Fos proteins on the line of v-myb transformed chicken monoblasts BM2. Both c-Fos and v-Fos proteins were ectopically expressed in BM2 cells. The v-Fos protein induced growth arrest in G0G1-phase of the BM2 cell cycle similarly as c-Jun protein. Interestingly, c-Fos leaved the cell cycle unaffected and rather induced programmed cell death. This effect of c-Fos protein was markedly enhanced in cells cultivated under serum-free conditions. In contrast to Jun proteins, no differentiation promoting effect of c-Fos and v-Fos protein was detected in BM2 cells. These results suggest differential roles of individual components of the AP-1 transcription factor in regulation of differentiation and programmed cell.

Links

• GA301/01/0040, research and development project: Name: Úloha proteinů Fos a Jun v monocytické diferenciaci stimulované kyselinou retinovou

Investor: Czech Science Foundation, The Role of Fos and Jun proteins in RA-driven monocytic differentiation

• GA301/03/1055, research and development project: Name: Studium molekulárních mechanismů způsobujících supresi v-Myb s využitím přístupů proteomiky

Investor: Czech Science Foundation, Study of molecular mechanisms causing v-Myb suppression using proteomics-based approach