2004
Determination of azidothymidine - an antiproliferative and virostatic drug by square wave voltammetry
VACEK, Jan, Zdeněk ANDRYSÍK, Libuše TRNKOVÁ a René KIZEKZákladní údaje
Originální název
Determination of azidothymidine - an antiproliferative and virostatic drug by square wave voltammetry
Název česky
Stanovení azidothymidinu- antiproliferačního a virostatického léku pomocí square wave voltametrie
Autoři
VACEK, Jan (203 Česká republika), Zdeněk ANDRYSÍK (203 Česká republika), Libuše TRNKOVÁ (203 Česká republika, garant) a René KIZEK (203 Česká republika)
Vydání
Electroanalysis, Germany, Weinheim, Wiley-VCH Verlag GmbH & Co. 2004, 1040-0397
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10405 Electrochemistry
Stát vydavatele
Německo
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 2.038
Kód RIV
RIV/00216224:14310/04:00011177
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000220047100008
Klíčová slova anglicky
Azidothymidine (AZT); Square wave voltammetry; Hanging mercury drop electrode (HMDE); DNA; Albumin; Urine; Serum; Whole blood; Keratinocytes (HaCaT line).
Štítky
Změněno: 15. 2. 2005 08:43, prof. RNDr. Libuše Trnková, CSc.
V originále
The azido-deoxythymidine (AZT, Zidovudine) is an antiproliferative and virostatic drug widely used in human immunodeficiency virus type 1 (HIV-1) infection treatment. With respect to side effects of high doses and a short half-life of AZT, a fast and simple detection method for this agent could be helpful. The aim of our study was to determine AZT levels in natural samples (urine, serum, whole blood, and cell cultures, such as the HaCaT line of keratinocytes) without their mineralization and/or purification, by means of electrochemical methods using hanging mercury drop electrode (HMDE). On this electrode, AZT undergoes irreversible reduction at the peak potential near Ep -1.1 V (vs. Ag/AgCl/3M KCl). Reduction AZT signals were measured by cyclic voltammetry (CV), differential pulse voltammetry (DPV), square wave voltammetry (SWV), and constant current chronopotentiometric stripping analysis (CPSA). In phosphate buffer (pH 8) the SWV yielded the best AZT signal with the detection limit of 1 nM. The determination of AZT concentration in biological materials is affected by electroactive components, such as proteins and DNA. For monitoring the influence of these compounds, AZT reduction was performed in the presence of 10 mg/mL calf thymus ssDNA and/or 100 mg/mL bovine serum albumin. In these cases, the detection limit increased to 0.25 mM. Also studied was the AZT concentration in keratinocyte cells was followed during cell cultivation. It has been shown that the SWV may be considered as a useful tool for the determination of AZT concentration in cell cultures, and for monitoring AZT pharmacokinetics.
Česky
Stanovení azidothymidinu-antiproliferačního a virostatického léku pomocí square wave voltametrie
Návaznosti
| GA203/02/0422, projekt VaV |
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| IAA1163201, projekt VaV |
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