Synthesis of mitochondrial uncoupling protein in brown adipocytes differentiated in cell culture.

KOPECKÝ, Jan, Marie BAUDYŠOVÁ, Franco ZANOTTI, Dagmar JANÍKOVÁ, Stanislav PAVELKA and Josef HOUŠTĚK. Synthesis of mitochondrial uncoupling protein in brown adipocytes differentiated in cell culture. Journal of Biological Chemistry [IF=6.698 (1989); SCI #cit.<61>]. 1990, vol. 265, 36,, p. 22204-22209. ISSN 0021-9258.

Basic information

Original name

Synthesis of mitochondrial uncoupling protein in brown adipocytes differentiated in cell culture.

Authors

KOPECKÝ, Jan, Marie BAUDYŠOVÁ, Franco ZANOTTI, Dagmar JANÍKOVÁ, Stanislav PAVELKA and Josef HOUŠTĚK

Edition

Journal of Biological Chemistry [IF=6.698 (1989); SCI #cit.<61>], 1990, 0021-9258

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

Keywords in English

biogenesis; brown adipose tissue; thermogenic mitochondria; uncoupling protein

Tags

Biogenesis, brown adipose tissue, thermogenic mitochondria, uncoupling protein

Tags

International impact, Reviewed

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Abstract

V originále

Differentiation of precursor cells isolated from mouse brown adipose tissue was studied in cell culture, in order to characterize the biogenesis of unique thermogenic mitochondria. Synthesis of mitochondrial uncoupling protein (UCP), F1-ATPase, and cytochrome oxidase was examined by L-[35S]methionine labeling and immunoblotting. In these cells, it was possible, by using norepinephrine, to induce specifically the synthesis of the UCP but not of F1-ATPase or cytochrome oxidase.