2004
Capillary Electrophoresis of Bacteriophage 812 Proteins Combined with MALDI Mass Spectrometry
PREISLER, Jan, Patrik VRÁBEL, Jaromíra NOVÁKOVÁ, Michal SPĚŠNÝ, Zbyněk ZDRÁHAL et. al.Základní údaje
Originální název
Capillary Electrophoresis of Bacteriophage 812 Proteins Combined with MALDI Mass Spectrometry
Název česky
Kapilární elektroforéza proteinů bakteriofága 812 kombinovaná s MALDI-hmotnostní spektrometrií
Autoři
PREISLER, Jan (203 Česká republika, garant, domácí), Patrik VRÁBEL (203 Česká republika, domácí), Jaromíra NOVÁKOVÁ (203 Česká republika, domácí), Michal SPĚŠNÝ (203 Česká republika, domácí), Zbyněk ZDRÁHAL (203 Česká republika, domácí), Hana KONEČNÁ (203 Česká republika, domácí), Pavel KRÁSENSKÝ (203 Česká republika, domácí), Luděk EYER (203 Česká republika, domácí), Jiří DOŠKAŘ (203 Česká republika), Roman PANTŮČEK (203 Česká republika, domácí), František FORET (203 Česká republika) a Karel KLEPÁRNÍK (203 Česká republika)
Vydání
Final Program and Book of Abstracts of 17th International Symposium on Microscale Separations and Capillary Electrophoresis, 2004
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
10406 Analytical chemistry
Stát vydavatele
Rakousko
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Kód RIV
RIV/00216224:14310/04:00011440
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
Capillary Electrophoresis; Proteins; Bacteriophage
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 11. 4. 2013 16:32, prof. Mgr. Jan Preisler, Ph.D.
V originále
The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. In this work, SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis.Additionally, we have proposed a new approach to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. Protein and peptide separations were performed with capillary electrophoresis (CE) and a new universal interface has been developed to couple CE with MALDI MS. A method of protein microdigestion on MALDI target is being studied and will be used for protein identification.
Česky
The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. In this work, SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis.Additionally, we have proposed a new approach to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. Protein and peptide separations were performed with capillary electrophoresis (CE) and a new universal interface has been developed to couple CE with MALDI MS. A method of protein microdigestion on MALDI target is being studied and will be used for protein identification. Anotace anglicky: The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. In this work, SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis.Additionally, we have proposed a new approach to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. Protein and peptide separations were performed with capillary electrophoresis (CE) and a new universal interface has been developed to couple CE with MALDI MS. A method of protein microdigestion on MALDI target is being studied and will be used for protein identification.
Návaznosti
| GA203/03/0515, projekt VaV |
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