2002
The study of potentially pathogenic lipophilic yeast Malassezia pachydermatis
DAVID, Marek, Miroslav GABRIEL a Marie KOPECKÁZákladní údaje
Originální název
The study of potentially pathogenic lipophilic yeast Malassezia pachydermatis
Autoři
DAVID, Marek (203 Česká republika, domácí), Miroslav GABRIEL (203 Česká republika, garant, domácí) a Marie KOPECKÁ (203 Česká republika, domácí)
Vydání
30nd Annual Conference on Yeasts, 2002
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
30000 3. Medical and Health Sciences
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14110/02:00011445
Organizační jednotka
Lékařská fakulta
Klíčová slova anglicky
lipophilic yeast; Malassezia pachydermatis; transmission electron microscopy; ultrastructure
Změněno: 29. 3. 2011 13:59, Mgr. Marek David, Ph.D.
Anotace
V originále
The potential human pathogen M. pachydermatis was studied to identify cytoskeleton as potential therapeutic target for antifungal agents. Light and transmission electron microscopy (TEM) confirmed the small cells (2,0-3,0 x 4,0-5,0 mm) and few intracellular structures. TEM confirmed the presence of thick multilayered cell wall that appeared to be a barrier for fluorescent antibodies. The higher concentration of detergent and lytical wall enzymes, the prolonged time of their and fluorescent antibodies applications seem necessary prerequisites to detect cytoskeleton. Preliminary results showed poor cytoskeletal structures. This report confirmed multilayered cell wall, the deep and helicoidal invaginations of plasma membrane and few intracellular structures. Cytoskeleton will be further studied by fluorescence and immunofluorescence and by TEM. The research was supported by grant No. 310/00/0391 of the Grant Agency of Czech Republic.
Návaznosti
| GA310/00/0391, projekt VaV |
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