Detailed Information on Publication Record
2003
Degradation of nitroaromatic compounds by members of the genus Rhodococcus
NAVRÁTILOVÁ, Jitka, Ludmila KOTOUČKOVÁ, Miroslav NĚMEC, Eva DURNOVÁ, Ivo SEDLÁČEK et. al.Basic information
Original name
Degradation of nitroaromatic compounds by members of the genus Rhodococcus
Name in Czech
Degradace nitroaromatických látek zástupci rodu Rhodococcus
Authors
NAVRÁTILOVÁ, Jitka (203 Czech Republic, guarantor), Ludmila KOTOUČKOVÁ (203 Czech Republic), Miroslav NĚMEC (203 Czech Republic), Eva DURNOVÁ (203 Czech Republic), Ivo SEDLÁČEK (203 Czech Republic) and Jiří NEČA (203 Czech Republic)
Edition
Abstract book of FEMS congress of european microbiologists, 2003
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10600 1.6 Biological sciences
Country of publisher
Slovenia
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/03:00010133
Organization unit
Faculty of Science
Keywords in English
nitroaromatic compounds; degradation; Rhodococcus
Změněno: 31/3/2010 11:32, prof. RNDr. Ivo Sedláček, CSc.
V originále
Nitroaromatic compounds are widely distributed in the environment. They are used as drugs, herbicides, pesticides, explosives, dyes and solvents and can be found as contaminants in waste waters, rivers and herbicide- or pesticide-treated soils. Nitroaromatics and products of their complete degradation have relatively high acute toxicity and some may be potential carcinogenic. A few of microorganisms are able to degrade these pollutants. Rhodococci, which are widely occurred in soil and sludge, have been shown to attack various xenobiotic compounds, including nitroaromatics. We have isolated 4 bacterial strains from soil by selective enrichment with nitroaromatic compounds in mineral medium. Two strains (J2J and J3) were isolated by enrichment with 4-nitrocatechol and two strains (J6 and J7) by enrichment with 4-nitroguaiacol. These strains were identified as members of the genus Rhodococcus on the base of a polyphasic taxonomic approach (morphology, biochemical and physiological characterisation, chemotaxonomic analysis, determination of partial sequence of genes for 16S rRNA). The nearest relatives were R. opacus and R. percolatus. The strains were tested for growth on a variety of nitroaromatic compounds such as 4 nitrocatechol (4-NC), 4-nitroguaiacol (4-NG), 5-nitroguaiacol (5-NG), 2-nitrophenol (2 NP), 3-nitrophenol (3-NP), 4-nitrophenol (4-NP), 2,4-dinitrobenzoic acid (2,4-DNBA), 4,5-dimethoxy-2-nitrobenzoic acid (4,5-dimethoxy-2 NBA) and 2,3-diphluoro-6 nitrophenol (2,3-diP-6 NP). Established degradations were particularly studied. There are some differences in degradation capability of strains but no one were able to degrade 2,4 DNBA, 4,5-dimethoxy-2 NBA and 2,3-diP-6 NP. Strain J3 was able to degrade 4-NC, 5-NG and 3-NP. Strain J6 has the broadest degradation spectrum. This strain was able to degrade 4-NC, 4-NG, 5-NG, 3-NP and 4-NP (all substrates in concentration 0,05 mM). Degradation of some nitroaromatic compounds (4-nitrocatechol, 4-nitroguaiacol and 4 nitrophenol) was monitored by the use of the automatic microbiological system BIOSCREEN C. Degradation of these compounds were tested in concentration range 0,025 mM to 0,1 mM. Degradations of nitroaromatic compounds were confirmed by the use of HPLC.
In Czech
Čtyři bakteriální kmeny byly izolovány metodou selektivního nahromadění na nitrovaných aromatických látkách. Dva z nich (J2 a J3) na 4-nitrokatecholu a další 2 (J6 a J7) na 4-nitrogujakolu. Kmeny byly podrobnou taxonomickou studií zařazeny do rodu Rhodococcus a podrobeny studiu degradační aktivity vůči spektru nitrovaných fenolů.
Links
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