LOCHMAN, Jan, Omar ŠERÝ and Vladimír MIKEŠ. The rapid identification of European Armillaria species from soil samples by nested PCR. FEMS Microbiology Letters. Amsterdam, Netherlands: Elsevier Science B. V., 2004, vol. 2004, No 237, p. 105-110. ISSN 0378-1097.
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Basic information
Original name The rapid identification of European Armillaria species from soil samples by nested PCR
Name in Czech Rychlá identifikace evropských druhů václavek z půdních vzorků pomocí metody nested PCR
Authors LOCHMAN, Jan (203 Czech Republic), Omar ŠERÝ (203 Czech Republic) and Vladimír MIKEŠ (203 Czech Republic, guarantor).
Edition FEMS Microbiology Letters, Amsterdam, Netherlands, Elsevier Science B. V. 2004, 0378-1097.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.840
RIV identification code RIV/00216224:14310/04:00010230
Organization unit Faculty of Science
UT WoS 000223031300014
Keywords in English Armillaria; PCR; soil
Tags Armillaria, PCR, soil
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Omar Šerý, Ph.D., učo 18120. Changed: 1/3/2007 17:10.
Abstract
New specific primers AR1 and AR2 were successfully used for the amplification of a specific part of internal transcribed spacer (ITS) of rDNA of Armillaria isolated from soil samples.The individual species were distinguished by restriction fragment length polymorphisms (RFLP) analysis with restriction endonucleases HinfI. For the first time, a method is described enabling the identification of Armillaria species within one day directly from soil samples without the need for previous isolation and cultivation of mycelium
Abstract (in Czech)
V rámci studie byly připraveny specifické primery AR1 a AR2, které slouží k amplifikaci specifické části oblasti ITS v rDNA václavek. Různé druhy václavek byly rozlišovány pomocí RFLP amplifikovaného fragmentu. Námi vyvinutá metoda umožňuje v průběhu jednoho dne určit druh václavky z půdního vzorku pomocí nested PCR a to bez zdlouhavé kultivace.
Links
MSM 143100005, plan (intention)Name: Strukturně-funkční vztahy biomolekul a jejich role v metabolismu
Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Structure-function Relationships and their role in the Metabolism
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