| JANICZEK, Oldřich, Zdeněk GLATZ, Michaela WIMMEROVÁ a Jitka PSOTOVÁ. Purification and some properties of isocitrate dehydrogenase from Paracoccus denitrificans. Preparative Biochemistry. New York: Marcel Dekker, Inc., 2004, roč. 34, č. 3, s. 279-289, 10 s. ISSN 0032-7484. |
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@article{557650,
author = {Janiczek, Oldřich and Glatz, Zdeněk and Wimmerová, Michaela and Psotová, Jitka},
article_location = {New York},
article_number = {3},
keywords = {Isocitrate dehydrogenase; purification; Paracoccus denitrificans},
language = {eng},
issn = {0032-7484},
journal = {Preparative Biochemistry},
title = {Purification and some properties of isocitrate dehydrogenase from Paracoccus denitrificans},
volume = {34},
year = {2004}
}
TY - JOUR
ID - 557650
AU - Janiczek, Oldřich - Glatz, Zdeněk - Wimmerová, Michaela - Psotová, Jitka
PY - 2004
TI - Purification and some properties of isocitrate dehydrogenase from Paracoccus denitrificans
JF - Preparative Biochemistry
VL - 34
IS - 3
SP - 279-289
EP - 279-289
PB - Marcel Dekker, Inc.
SN - 00327484
KW - Isocitrate dehydrogenase
KW - purification
KW - Paracoccus denitrificans
N2 - NADP-dependent isocitrate dehydrogenase (ICDH) from the bacterium Paracoccus denitrificans was purified to homogeneity. The purification procedure involved ammonium sulphate fractionation, ion exchange chromatography, and gel permeation chromatography. The purity of the enzyme was checked by polyacrylamide gel electrophoresis. ICDH is a dimer composed of two probably identical subunits of relative molecular weight 90000. The pH optimum of the enzyme reaction in the direction of substrate oxidation was found to be 5.6; the presence of Mn (2+) is essential for enzyme activity.
ER -
JANICZEK, Oldřich, Zdeněk GLATZ, Michaela WIMMEROVÁ a Jitka PSOTOVÁ. Purification and some properties of isocitrate dehydrogenase from Paracoccus denitrificans. \textit{Preparative Biochemistry}. New York: Marcel Dekker, Inc., 2004, roč.~34, č.~3, s.~279-289, 10 s. ISSN~0032-7484.
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