Synthesis and DNA conformational changes of non-covalent
polynuclear platinum complexes

J 2004

Synthesis and DNA conformational changes of non-covalent polynuclear platinum complexes

QU, Yun, Amanda HARRIS, Andrea PETZ, Peyman KABOLIZADEH, Hana PENAZOVA et. al.

Basic information

Original name

Synthesis and DNA conformational changes of non-covalent polynuclear platinum complexes

Name in Czech

Syntéza a konformační změny DNA vyvolané nekovalentně se vážícími vícejadernými koordinačními sloučeninami platiny

Authors

QU, Yun (156 China), Amanda HARRIS (840 United States of America), Andrea PETZ (840 United States of America), Peyman KABOLIZADEH (840 United States of America), Hana PENAZOVA (203 Czech Republic, guarantor) and Nicholas FARRELL (372 Ireland)

Edition

Journal of Inorganic Biochemistry, New York, USA, Elsevier, 2004, 0162-0134

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10610 Biophysics

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.225

RIV identification code

RIV/00216224:14410/04:00030654

Organization unit

Faculty of Education

UT WoS

000224599100007

Keywords in English

Non-covalent DNA binding; Polynuclear platinum

Abstract

ORIG CZ

V originále

Polynuclear platinum compounds demonstrate many novel phenomena in their interactions with DNA and proteins as well as novel anti-cancer activities. Previous studies indicated that the high positive charge and the non-coordinated Ącentral linkerĄą of the polynuclear compounds could have major contributions to these features. Therefore, a series of non-covalent polynuclear platinum complexes, [ Pt(NH3)3 2-ŚĚ-Y]n+ (Y = polyamine linker or trans-Pt(NH3)2(H2N(CH2)6NH2)2 ) was synthesized and the DNA interactions of these platinum complexes were investigated. The conformational changes induced by these compounds in polymer DNA were studied by circular dichroism and the reversibility of the transition was tested by subsequent titration with the DNA intercalating agent ethidium bromide (EtBr). Fluorescent quenching was also used to assess the ability of EtBr to intercalate into A and Z-DNA induced by the compounds. The non-covalent polynuclear platinum complexes induced both B Ąú A and B Ąú Z conformational changes in polymer DNA. These conformational changes were partially irreversible. The platinum compound with the spermidine linker, [ Pt(NH3)3 2-ŚĚ-spermidine-N1,N8]Cl5 Ą 2H2O, is more efficient in inducing the conformational changes of DNA and it is less reversible than complexes with other linkers. The melting point study showed that the non-covalent polynuclear platinum complexes stabilized the duplex DNA and the higher the electrical charge of the complexes the greater the stabilization observed.

In Czech

Článek popisuje syntézu několika vícejaderných koordinačních sloučenin Pt a uvádí výsledky předběžného výzkumu jejich interakce s DNA.

Citovat

QU, Yun, Amanda HARRIS, Andrea PETZ, Peyman KABOLIZADEH, Hana PENAZOVA and Nicholas FARRELL. Synthesis and DNA conformational changes of non-covalent polynuclear platinum complexes. Journal of Inorganic Biochemistry. New York, USA: Elsevier, 2004, vol. 98, No 10, p. 1591-1598. ISSN 0162-0134.

@article{557861,
   author = {Qu, Yun and Harris, Amanda and Petz, Andrea and Kabolizadeh, Peyman and Penazova, Hana and Farrell, Nicholas},
   article_location = {New York, USA},
   article_number = {10},
   keywords = {Non-covalent DNA binding; Polynuclear platinum},
   language = {eng},
   issn = {0162-0134},
   journal = {Journal of Inorganic Biochemistry},
   title = {Synthesis and DNA conformational changes of non-covalent polynuclear platinum complexes},
   volume = {98},
   year = {2004}
}

TY  - JOUR
ID  - 557861
AU  - Qu, Yun - Harris, Amanda - Petz, Andrea - Kabolizadeh, Peyman - Penazova, Hana - Farrell, Nicholas
PY  - 2004
TI  - Synthesis and DNA conformational changes of non-covalent polynuclear platinum complexes
JF  - Journal of Inorganic Biochemistry
VL  - 98
IS  - 10
SP  - 1591-1598
EP  - 1591-1598
PB  - Elsevier
SN  - 01620134
KW  - Non-covalent DNA binding
KW  - Polynuclear platinum
N2  - Polynuclear platinum compounds demonstrate many novel phenomena in their interactions with DNA and proteins as well as novel anti-cancer activities. Previous studies indicated that the high positive charge and the non-coordinated Ącentral linkerĄą of the polynuclear compounds could have major contributions to these features. Therefore, a series of non-covalent polynuclear platinum complexes, [ Pt(NH3)3 2-ŚĚ-Y]n+ (Y = polyamine linker or trans-Pt(NH3)2(H2N(CH2)6NH2)2 ) was synthesized and the DNA interactions of these platinum complexes were investigated. The conformational changes induced by these compounds in polymer DNA were studied by circular dichroism and the reversibility of the transition was tested by subsequent titration with the DNA intercalating agent ethidium bromide (EtBr). Fluorescent quenching was also used to assess the ability of EtBr to intercalate into A and Z-DNA induced by the compounds. The non-covalent polynuclear platinum complexes induced both B Ąú A and B Ąú Z conformational changes in polymer DNA. These conformational changes were partially irreversible. The platinum compound with the spermidine linker, [ Pt(NH3)3 2-ŚĚ-spermidine-N1,N8]Cl5 Ą 2H2O, is more efficient in inducing the conformational changes of DNA and it is less reversible than complexes with other linkers. The melting point study showed that the non-covalent polynuclear platinum complexes stabilized the duplex DNA and the higher the electrical charge of the complexes the greater the stabilization observed.
ER  -

QU, Yun, Amanda HARRIS, Andrea PETZ, Peyman KABOLIZADEH, Hana PENAZOVA and Nicholas FARRELL. Synthesis and DNA conformational changes of non-covalent polynuclear platinum complexes. \textit{Journal of Inorganic Biochemistry}. New York, USA: Elsevier, 2004, vol.~98, No~10, p.~1591-1598. ISSN~0162-0134.

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