Nitric oxide oscillations in Paracoccus denitrificans: the
effects of environmental factors and of segregating nitrite
reductase and nitric oxide reductase into separate cells

J 2004

Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells

KUŇÁK, Michal, Igor KUČERA a Rob J.M. VAN SPANNING

Základní údaje

Originální název

Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells

Název česky

Oscilace oxidu dusnatého u Paracoccus denitrificans: vliv faktorů prostředí o oddělení nitritreduktasy a reduktasy oxidu dusnatého do různých buněk

Autoři

KUŇÁK, Michal (203 Česká republika), Igor KUČERA (203 Česká republika, garant) a Rob J.M. VAN SPANNING (528 Nizozemské království)

Vydání

Archives of biochemistry and biophysics, New York, Academic Press, 2004, 0003-9861

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.657

Kód RIV

RIV/00216224:14310/04:00010556

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000223563600015

Klíčová slova anglicky

denitrification; nitrite reductase; nitric oxide reductase; substrate inhibition; concentration oscillations

Štítky

concentration oscillations, denitrification, nitric oxide reductase, nitrite reductase, substrate inhibition

Změněno: 10. 2. 2005 12:30, prof. RNDr. Igor Kučera, DrSc.

Anotace

ORIG CZ

V originále

Nitric oxide is a denitrification intermediate which is produced from nitrite and then further converted via nitrous oxide to nitrogen. Here, the effect of low concentrations of the protonophore carbonylcyanide m-chlorophenylhydrazone on the time courses for dissolved gases was examined. While NO was found to oscillate, N(2)O only increased gradually as the reduction of nitrite progressed. The frequency and shape of protonophore-induced NO oscillations were influenced by temperature and the concentration of electron donor N,N,N',N'-tetramethyl-p-phenylene diamine (TMPD) in a manner compatible with the observed differential effects on the two involved enzyme activities. We demonstrated the existence of a pH interval, where [NO] oscillates even without uncoupler addition. Occurrence of nitric oxide oscillations in mixtures of a nitrite reductase mutant with a nitric oxide reductase mutant suggests that they cannot be due to a competition of the enzymes for redox equivalents from one common respiratory chain.

Česky

Oxid dusnatý je meziprodukt denitrifikace, který vzniká z dusitanu a je dále přeměňován přes oxid dusný na dusík. Byl sledován vliv nízkých koncentrací protonoforu karbonylkyanid-m-chlorfenylhydrazonu na časový průběh koncentrací rozpuštěných plynů. Zatímco u NO byly zjištěny oscilace, N2O se postupně hromadil. Frekvence a tvar oscilací NO vyvolaných protonoforem byly ovlivňovány teplotou a koncentrací elektronového donoru TMPD v souladu s pozorovaným působením na aktivity jednotlivých enzymů. Ukázali jsme existenci intervalu pH ve kterém NO osciluje dokonce bez přidání rozpojovače. Výskyt oscilací NO ve směsi mutant ukazuje, že nemohou být způsobeny kompeticí obou enzymů o redukční ekvivalenty z jednoho společného respiračního řetězce.

Návaznosti

MSM 143100005, záměr

Název: Strukturně-funkční vztahy biomolekul a jejich role v metabolismu

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Strukturně-funkční vztahy biomolekul a jejich role v metabolismu

Citovat

KUŇÁK, Michal, Igor KUČERA a Rob J.M. VAN SPANNING. Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells. Archives of biochemistry and biophysics. New York: Academic Press, 2004, roč. 429, č. 2, s. 237-243. ISSN 0003-9861.

@article{561459,
   author = {Kuňák, Michal and Kučera, Igor and Van Spanning, Rob J.M.},
   article_location = {New York},
   article_number = {2},
   keywords = {denitrification; nitrite reductase; nitric oxide reductase; substrate inhibition; concentration oscillations},
   language = {eng},
   issn = {0003-9861},
   journal = {Archives of biochemistry and biophysics},
   title = {Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells},
   url = {http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WB5-4D0Y364-2&_coverDate=09%2F15%2F2004&_alid=223287397&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=6701&_sort=d&view=c&_acct=C000045159&_version=1&_urlVersion=0&_userid=835458&md5=e269cd2afbc12bda4bc9},
   volume = {429},
   year = {2004}
}

TY  - JOUR
ID  - 561459
AU  - Kuňák, Michal - Kučera, Igor - Van Spanning, Rob J.M.
PY  - 2004
TI  - Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells
JF  - Archives of biochemistry and biophysics
VL  - 429
IS  - 2
SP  - 237-243
EP  - 237-243
PB  - Academic Press
SN  - 00039861
KW  - denitrification
KW  - nitrite reductase
KW  - nitric oxide reductase
KW  - substrate inhibition
KW  - concentration oscillations
UR  - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WB5-4D0Y364-2&_coverDate=09%2F15%2F2004&_alid=223287397&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=6701&_sort=d&view=c&_acct=C000045159&_version=1&_urlVersion=0&_userid=835458&md5=e269cd2afbc12bda4bc9
N2  - Nitric oxide is a denitrification intermediate which is produced from nitrite and then further converted via nitrous oxide to nitrogen. Here, the effect of low concentrations of the protonophore carbonylcyanide m-chlorophenylhydrazone on the time courses for dissolved gases was examined. While NO was found to oscillate, N(2)O only increased gradually as the reduction of nitrite progressed. The frequency and shape of protonophore-induced NO oscillations were influenced by temperature and the concentration of electron donor N,N,N',N'-tetramethyl-p-phenylene diamine (TMPD) in a manner compatible with the observed differential effects on the two involved enzyme activities. We demonstrated the existence of a pH interval, where [NO] oscillates even without uncoupler addition. Occurrence of nitric oxide oscillations in mixtures of a nitrite reductase mutant with a nitric oxide reductase mutant suggests that they cannot be due to a competition of the enzymes for redox equivalents from one common respiratory chain.
ER  -

KUŇÁK, Michal, Igor KUČERA a Rob J.M. VAN SPANNING. Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells. \textit{Archives of biochemistry and biophysics}. New York: Academic Press, 2004, roč.~429, č.~2, s.~237-243. ISSN~0003-9861.

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