KUBEK, Tomáš, Michal KÝR, Pavel HANINEC, Filip ŠÁMAL a Petr DUBOVÝ. Morphological evidence of collateral sprouting of intact afferent and motor axons of the rat ulnar nerve by one type of tracer molecule. Annals of Anatomy. Elsevier, 2004, roč. 186, č. 3, s. 231-234. ISSN 0940-9602. |
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@article{561594, author = {Kubek, Tomáš and Kýr, Michal and Haninec, Pavel and Šámal, Filip and Dubový, Petr}, article_number = {3}, keywords = {afferent; efferent; sprouts}, language = {eng}, issn = {0940-9602}, journal = {Annals of Anatomy}, title = {Morphological evidence of collateral sprouting of intact afferent and motor axons of the rat ulnar nerve by one type of tracer molecule}, volume = {186}, year = {2004} }
TY - JOUR ID - 561594 AU - Kubek, Tomáš - Kýr, Michal - Haninec, Pavel - Šámal, Filip - Dubový, Petr PY - 2004 TI - Morphological evidence of collateral sprouting of intact afferent and motor axons of the rat ulnar nerve by one type of tracer molecule JF - Annals of Anatomy VL - 186 IS - 3 SP - 231-234 EP - 231-234 PB - Elsevier SN - 09409602 KW - afferent KW - efferent KW - sprouts N2 - In our experimental paradigm we sutured distal stump of transected musculocutaneous nerve to an intact ulnar nerve of the rat by the end-to-side fashion. We demonstrated the formation of collateral sprouts from intact afferent and motor axons by application of one type of molecule conjugated by two different fluorophores (Fluoro-Ruby and Fluoro-Emerald). Fluoro-Ruby and Fluoro-Emerald were applied distal to end-to-side suture into fresh cut ends of the ulnar and musculocutaneous nerves, respectively. Formation of collateral sprouts was evidenced by findings of mixed (orange) fluorescence labeling of spinal motoneurons and dorsal root ganglia neurons. Colocalization of two different tracers retrogradely transported to the neurons was verified by green and red profiles analyzed by means of image analysis system. Our results unequivocally demonstrate that a nerve stump attached to an intact nerve can induce collateral sprouting of both afferent and motor axons. ER -
KUBEK, Tomáš, Michal KÝR, Pavel HANINEC, Filip ŠÁMAL a Petr DUBOVÝ. Morphological evidence of collateral sprouting of intact afferent and motor axons of the rat ulnar nerve by one type of tracer molecule. \textit{Annals of Anatomy}. Elsevier, 2004, roč.~186, č.~3, s.~231-234. ISSN~0940-9602.
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