2004
Morphological evidence of collateral sprouting of intact afferent and motor axons of the rat ulnar nerve by one type of tracer molecule
KUBEK, Tomáš; Michal KÝR; Pavel HANINEC; Filip ŠÁMAL; Petr DUBOVÝ et. al.Basic information
Original name
Morphological evidence of collateral sprouting of intact afferent and motor axons of the rat ulnar nerve by one type of tracer molecule
Name in Czech
Morfogický důkaz tvorby kolaterál intaktních aferentních a motorických axonů v nervus ulnaris potkana pomocí jednoho typu transportované molekuly.
Authors
KUBEK, Tomáš (203 Czech Republic); Michal KÝR (203 Czech Republic); Pavel HANINEC (203 Czech Republic); Filip ŠÁMAL (203 Czech Republic) and Petr DUBOVÝ (203 Czech Republic, guarantor)
Edition
Annals of Anatomy, Elsevier, 2004, 0940-9602
Other information
Language
English
Type of outcome
Article in a journal
Field of Study
10600 1.6 Biological sciences
Country of publisher
Germany
Confidentiality degree
is not subject to a state or trade secret
Impact factor
Impact factor: 0.540
RIV identification code
RIV/00216224:14110/04:00010569
Organization unit
Faculty of Medicine
UT WoS
000222263500006
Keywords in English
afferent; efferent; sprouts
Tags
International impact, Reviewed
Changed: 26/6/2009 10:52, prof. RNDr. Petr Dubový, CSc.
In the original language
In our experimental paradigm we sutured distal stump of transected musculocutaneous nerve to an intact ulnar nerve of the rat by the end-to-side fashion. We demonstrated the formation of collateral sprouts from intact afferent and motor axons by application of one type of molecule conjugated by two different fluorophores (Fluoro-Ruby and Fluoro-Emerald). Fluoro-Ruby and Fluoro-Emerald were applied distal to end-to-side suture into fresh cut ends of the ulnar and musculocutaneous nerves, respectively. Formation of collateral sprouts was evidenced by findings of mixed (orange) fluorescence labeling of spinal motoneurons and dorsal root ganglia neurons. Colocalization of two different tracers retrogradely transported to the neurons was verified by green and red profiles analyzed by means of image analysis system. Our results unequivocally demonstrate that a nerve stump attached to an intact nerve can induce collateral sprouting of both afferent and motor axons.
In Czech
K experimntům byl použit vlastní model end-to-side anastomosy, ve kterém byl distální pahýl přerušeného n. musculocutaneus našit do perineuriálního okna v n. ulnaris laboratorního potkana.
Links
| NF6658, research and development project |
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