Detailed Information on Publication Record
2004
Cytological study of DNA methylation and histone methylation patterns in human cell lines
SKALNÍKOVÁ, Magdalena, Eva BÁRTOVÁ, Stanislav KOZUBEK and Michal KOZUBEKBasic information
Original name
Cytological study of DNA methylation and histone methylation patterns in human cell lines
Name in Czech
Cytologická studie chování DNA metylace a histonové metylace v lidských buněčných liniích
Authors
SKALNÍKOVÁ, Magdalena (203 Czech Republic), Eva BÁRTOVÁ (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic) and Michal KOZUBEK (203 Czech Republic, guarantor)
Edition
First Edition 2004. Brno, Biophysics of the Genome, p. 72-75, 4 pp. 2004
Publisher
Masaryk University
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
Genetics and molecular biology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14330/04:00009706
Organization unit
Faculty of Informatics
ISBN
80-210-3560-9
Keywords in English
cytometry
Tags
Tags
International impact
Změněno: 29/10/2010 14:31, prof. RNDr. Michal Kozubek, Ph.D.
V originále
Epigenetic processes are defined as heritable changes in genome function that occur without a change in DNA sequence. Gene expression, chromosome segregation, DNA replication, repair, and recombination all act, not on DNA alone, but on the chromatin template. DNA methylation, along with histone lysine methylation, establishes the framework for long-term epigenetic maintenance. The discovery that enzymes can (re)organise chromatin into accessible and inaccessible configurations revealed epigenetic mechanisms that considerably extend the information potential of the genetic code. In mammals, heterochromatin is characterised by DNA methylation at CpG dinucleotides and methylation at lysine 9 of histone 3 (H3- K9), whereas euchromatin is associated with methylation at lysine 4 of histone 3 (H3-K4). In our experiments, the detection of DNA methylated regions at chromosomal level was performed using indirect immunofluorescence technique combined with FISH. The selected metaphase chromosomes HSA 2 were determined using FISH. Sequential 5-methylcytosin rich regions were visualised using immunofluorescent labelling in retinoblastoma cell line Y79 and in leukemic cell line K562. Our model study is partly focused on the global histone H3-K4 and H3-K9 trimethylation studied by qualitative immunohistochemistry in retinoblastoma cells Y79 and leukemic cells K562. Our results highlight the importance of the relationships between histone modifications and DNA methylation, however the mutual connection between individual epigenetic hereditary mechanisms has not been made clear yet.
In Czech
Cytologická studie chování DNA metylace a histonové metylace v lidských buněčných liniích.
Links
| GA202/04/0907, research and development project |
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| GP202/03/D033, research and development project |
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| IAA5004306, research and development project |
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| MSM 143300002, plan (intention) |
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| NC6987, research and development project |
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| 1A8241, research and development project |
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