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@inproceedings{563815,
author = {Vařecha, Miroslav and Amrichová, Jana and Ondřej, Vladan and Lukášová, Emilie and Kozubek, Stanislav and Matula, Pavel and Matula, Petr and Kozubek, Michal},
address = {Brno},
booktitle = {Biophysics of the Genome},
edition = {First Edition 2004},
keywords = {living cell},
language = {eng},
location = {Brno},
isbn = {80-210-3560-9},
pages = {76-82},
publisher = {Masaryk University},
title = {In vivo imaging of cell interior using automated high-resolution cytometry},
year = {2004}
}
TY - JOUR
ID - 563815
AU - Vařecha, Miroslav - Amrichová, Jana - Ondřej, Vladan - Lukášová, Emilie - Kozubek, Stanislav - Matula, Pavel - Matula, Petr - Kozubek, Michal
PY - 2004
TI - In vivo imaging of cell interior using automated high-resolution cytometry
PB - Masaryk University
CY - Brno
SN - 8021035609
KW - living cell
N2 - The fluorescent proteins are important innovation in the field of cell biology and in vivo experiments that use fluorescent proteins bring new interesting results from a different point of view than in vitro and in situ experiments. This presentation will introduce our automated 2D/3D high-resolution time-lapse image acquisition and analysis of living cells transfected with plasmids encoding fusion proteins or dyed with fluorescence probes. One of our in vivo projects, we will mention, is focused on the study of mitochondrial and nuclear apoptotic processes in living cells. Another in vivo project concentrates on the study of topography of telomeres and incidence of telomere-association phenomenon in various types of human tumor and healthy cells. In our experiments, cells are stably or transiently transfected with plasmid DNAs coding fusion mitochondrial apoptogenic or telomere-binding proteins with fluorescent proteins.
ER -
VAŘECHA, Miroslav, Jana AMRICHOVÁ, Vladan ONDŘEJ, Emilie LUKÁŠOVÁ, Stanislav KOZUBEK, Pavel MATULA, Petr MATULA and Michal KOZUBEK. In vivo imaging of cell interior using automated high-resolution cytometry. In \textit{Biophysics of the Genome}. First Edition 2004. Brno: Masaryk University, 2004, p.~76-82. ISBN~80-210-3560-9.
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