J 2003

Biochemical analysis of soil organic matter and microbial biomass composition - a pilot study

HOFMAN, Jakub and Ladislav DUŠEK

Basic information

Original name

Biochemical analysis of soil organic matter and microbial biomass composition - a pilot study

Name in Czech

Biochemická analýza složení půdní organické hmoty a mikrobiální biomasy - pilotní studie

Authors

HOFMAN, Jakub (203 Czech Republic, guarantor) and Ladislav DUŠEK (203 Czech Republic)

Edition

European Journal of Soil Biology, 2003, 1164-5563

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

40104 Soil science

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.830

RIV identification code

RIV/00216224:14310/03:00010826

Organization unit

Faculty of Science

UT WoS

000187082900006

Keywords in English

soil microbial biomass

Tags

International impact, Reviewed
Změněno: 24/2/2010 20:46, prof. RNDr. Jakub Hofman, Ph.D.

Abstract

V originále

Biochemical composition of both intracellular (biomass) and extracellular soil organic matter was determined after extraction with 0.5 M K2SO4. Extractable carbon, hexoses, pentoses, total reducing sugars, ninhydrin-reactive nitrogen, proteins and DNA content were colorimetrically determined. The objective of the pilot study was to examine the information potential included in newly measured biochemical characteristics, their environmental variance and the relationships with main soil properties. Correlation analysis and PCA showed independence between biochemical parameters and physico-chemical properties of the soil. Thus, the parameters characterising biochemical composition of the soil biomass and extracellular matter seem to bring new information about the soils beyond the physico-chemical parameters. They also seem to reveal a more detailed view on microbial biomass or extracellular organic matter pool than Cbio or Cext alone, respectively. The variance which occurred in biochemical characteristics displayed also a high discrimination potential between the defined soil categories. Three types of indices were newly proposed: index I ("substrate quantity index") - the biomass-specific amount of the extracellular organic compounds, index II ("immobilisation ratio") - the portion of the organic compound immobilised in microbial biomass, and index III ("substrate quality index") - the extracellular organic compound content related to extracellular organic carbon. The indices displayed a higher potential than both soil biotic and abiotic parameters to discriminate soil characters and soil types.

In Czech

Biochemická analýza složení půdní organické hmoty a mikrobiální biomasy - pilotní studie

Links

GA526/98/1147, research and development project
Name: Diversita a funkční analýza půdních mikrobiálních společenstev v terestrických ekosystémech kontaminovaných persistentními organickými polutanty (POP)
Investor: Czech Science Foundation, Diversity and functional analysis of soil microbial communities in terrestrial ecosystems polluted by persistent organic pollutants (POPs)
MSM 141100003, plan (intention)
Name: Zevní prostředí - karcinogeneze - onkologie
Investor: Ministry of Education, Youth and Sports of the CR, Environment - carcinogenesis - oncology
SI/340/1/00, research and development project
Name: Region specifický přístup v analýze ekologických rizik - metodika zhodnocení ve vztahu k přírodním katastrofám.