Detailed Information on Publication Record
2005
Image acquisition and its automation in fluorescence microscopy
KOZUBEK, MichalBasic information
Original name
Image acquisition and its automation in fluorescence microscopy
Name in Czech
Snímání obrazu a jeho automatizace ve fluorescenční mikroskopii
Authors
KOZUBEK, Michal (203 Czech Republic, guarantor)
Edition
Dordrecht, From cells to proteins: Imaging nature across dimensions, p. 227-270, 44 pp. NATO Science Series, 2005
Publisher
Springer
Other information
Language
English
Type of outcome
Kapitola resp. kapitoly v odborné knize
Field of Study
20200 2.2 Electrical engineering, Electronic engineering, Information engineering
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14330/05:00013579
Organization unit
Faculty of Informatics
ISBN
1-4020-3615-9
UT WoS
000235038500013
Keywords in English
image acquisition; fluorescence microscopy; automated microscopy; image cytometry; FISH
Tags
International impact, Reviewed
Změněno: 7/5/2010 15:10, prof. RNDr. Michal Kozubek, Ph.D.
V originále
Fluorescence microscopy is presently the main tool for the investigation of structure and function of cells and their components. Unfortunately, this type of microscopy suffers from low light levels and consequent high noise levels. This poses big challenge for the image acquisition process. The selection of proper hardware and software parts of the whole set-up (microscope, light detector, driving computer) as well as optimization of the imaging conditions are highly non-trivial tasks that require deep understanding of the laws of physics and mutual dependences of a number of phenomena. This chapter brings insight into these laws and dependencies and provides the reader with a number of hints for the selection of appropriate system components as well as for the optimization of their performance. Special attention is paid to automation in fluorescence microscopy that is absolutely indispensable if multi-dimensional data are to be acquired.
In Czech
Kapitola pojednává o snímání obrazu a jeho automatizaci ve fluorescenční mikroskopii.
Links
| MSM 143300002, plan (intention) |
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