| KLEJDUS, Borivoj, Jan VACEK, Vojtech ADAM, Josef ZEHNÁLEK, René KIZEK, Libue TRNKOVÁ a Vlastimil KUBÁN. Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection. Journal of Chromatography B. Amsterdam, The Netherlands: Elsevier, 2004, roč. 806, č. 1, s. 101 - 111. ISSN 1570-0232. |
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@article{567226,
author = {Klejdus, Borivoj and Vacek, Jan and Adam, Vojtech and Zehnálek, Josef and Kizek, René and Trnková, Libue and Kubán, Vlastimil},
article_location = {Amsterdam, The Netherlands},
article_number = {1},
keywords = {Isoflavones; HPLC; electrochemical detection},
language = {eng},
issn = {1570-0232},
journal = {Journal of Chromatography B},
title = {Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection},
volume = {806},
year = {2004}
}
TY - JOUR
ID - 567226
AU - Klejdus, Borivoj - Vacek, Jan - Adam, Vojtech - Zehnálek, Josef - Kizek, René - Trnková, Libue - Kubán, Vlastimil
PY - 2004
TI - Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection
JF - Journal of Chromatography B
VL - 806
IS - 1
SP - 101 - 111
EP - 101 - 111
PB - Elsevier
SN - 15700232
KW - Isoflavones
KW - HPLC
KW - electrochemical detection
N2 - A highly sensitive high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) was developed for the determination of isoflavones. Electrochemical behaviour of daidzein and genistein was studied on carbon paste electrode (CPE) by adsorptive transfer stripping square wave voltammetry. The obtained electrochemical results were used for the development of HPLC-ED method. Furthermore, isoflavones were separated on an Atlantis dC18 column using a mobile phase consisting of acetonitrile (solvent A) and 0.15M acetate buffer of pH 5.5 (solvent B) at a flow rate 0.4 mL/min. A linear gradient profile (solvent B) was at 02 min 87%; 22 min 60%; 27 min 50%; 31 min 45%; 47 min 87%. Full scan of multi-channel coulometric detection was tested and optimal potential at 450mV was chosen for our purposes. Calibration curves were linear (daidzein R2 = 0.9993 and genistein R2 = 0.9987). The detection limit for daidzein/genistein was 480/394 pg/mL (1.8/1.5 nM) and per column 2.4/1.9 pg. Isoflavones extracted from soybean products (farina, meat, milk) by the accelerated solvent extraction (ASE) procedure and isoflavones present in human urine were determined the HPLC-ED method.
ER -
KLEJDUS, Borivoj, Jan VACEK, Vojtech ADAM, Josef ZEHNÁLEK, René KIZEK, Libue TRNKOVÁ a Vlastimil KUBÁN. Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection. \textit{Journal of Chromatography B}. Amsterdam, The Netherlands: Elsevier, 2004, roč.~806, č.~1, s.~101 - 111. ISSN~1570-0232.
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