Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection

KLEJDUS, Borivoj, Jan VACEK, Vojtech ADAM, Josef ZEHNÁLEK, René KIZEK, Libue TRNKOVÁ and Vlastimil KUBÁN. Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection. Journal of Chromatography B. Amsterdam, The Netherlands: Elsevier, 2004, vol. 806, No 1, p. 101 - 111. ISSN 1570-0232.

Basic information

• Original name: Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection
• Name in Czech: Stanovení isoflavon v sojových bobech a v lidské moi za použití HPLC s elektrochemickou detekcí
• Authors: KLEJDUS, Borivoj (203 Czech Republic), Jan VACEK (203 Czech Republic), Vojtech ADAM (203 Czech Republic), Josef ZEHNÁLEK (203 Czech Republic), René KIZEK (203 Czech Republic), Libue TRNKOVÁ (203 Czech Republic, guarantor) and Vlastimil KUBÁN (203 Czech Republic).
• Edition: Journal of Chromatography B, Amsterdam, The Netherlands, Elsevier, 2004, 1570-0232.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 2.176
• RIV identification code: RIV/00216224:14310/04:00011226
• Organization unit: Faculty of Science
• UT WoS: 000221945700004
• Keywords in English: Isoflavones; HPLC; electrochemical detection
• Tags: electrochemical detection, HPLC, Isoflavones

Abstract

A highly sensitive high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) was developed for the determination of isoflavones. Electrochemical behaviour of daidzein and genistein was studied on carbon paste electrode (CPE) by adsorptive transfer stripping square wave voltammetry. The obtained electrochemical results were used for the development of HPLC-ED method. Furthermore, isoflavones were separated on an Atlantis dC18 column using a mobile phase consisting of acetonitrile (solvent A) and 0.15M acetate buffer of pH 5.5 (solvent B) at a flow rate 0.4 mL/min. A linear gradient profile (solvent B) was at 02 min 87%; 22 min 60%; 27 min 50%; 31 min 45%; 47 min 87%. Full scan of multi-channel coulometric detection was tested and optimal potential at 450mV was chosen for our purposes. Calibration curves were linear (daidzein R2 = 0.9993 and genistein R2 = 0.9987). The detection limit for daidzein/genistein was 480/394 pg/mL (1.8/1.5 nM) and per column 2.4/1.9 pg. Isoflavones extracted from soybean products (farina, meat, milk) by the accelerated solvent extraction (ASE) procedure and isoflavones present in human urine were determined the HPLC-ED method.

Abstract (in Czech)

Stanovení isoflavon v sojových bobech a v lidské moi za použití HPLC s elektrochemickou detekcí

Links

• GA203/02/0422, research and development project: Name: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí

Investor: Czech Science Foundation, New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry

• IAA1163201, research and development project: Name: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin

Investor: Academy of Sciences of the Czech Republic, Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.