2004
Využití katalytických reakcí na rtuťových elektrodách pro elektrochemické stanovení metalothioneinu
KIZEK, René, Jan VACEK, Libuše TRNKOV, Bořivoj KLEJDUS, Ladislav HAVEL et. al.Základní údaje
Originální název
Využití katalytických reakcí na rtuťových elektrodách pro elektrochemické stanovení metalothioneinu
Název česky
Využití katalytických reakcí na rtuťových elektrodách pro elektrochemické stanovení metalothioneinu
Název anglicky
Application of catalytic reactions on a mercury electrode for electrochemical detection of metallothioneins
Autoři
KIZEK, René (203 Česká republika), Jan VACEK (203 Česká republika), Libuše TRNKOV (203 Česká republika, garant), Bořivoj KLEJDUS (203 Česká republika) a Ladislav HAVEL (203 Česká republika)
Vydání
Chemick Listy, Praha, esk spolenost chemick, 2004, 0009-2770
Další údaje
Jazyk
čeština
Typ výsledku
Článek v odborném periodiku
Obor
10405 Electrochemistry
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 0.348
Kód RIV
RIV/00216224:14310/04:00011232
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000221682700003
Klíčová slova anglicky
metallothionein; electrochemical analysis; catalytic reactions; peak H; Brdicka reaction
Změněno: 15. 2. 2005 20:16, prof. RNDr. Libuše Trnková, CSc.
V originále
Metallothioneins (MTs) belong to a group of oligo- and polypeptides which play an important role in the metabolism of metals in animals, plants and microorganisms. The metabolic function of MTs consists not only in detoxication of an organism but also in the homeostasis of essential metals. This paper reports a novel approach to electroanalysis of MTs. The electrochemical determination of MTs is based on catalytic processes which proceed at very negative potentials on mercury electrodes (from -1.7 V to -1.9 V vs. Ag/AgCl/KCl). These processes accompanied by evolution of hydrogen from supporting electrolyte components, include the Heyrovsky presodium wave and/or Brdicka reaction. It was found that SH groups present in MTs are responsible for catalytic processes. The catalytic signal of a MT at nanomolar concentrations can be detected on mercury electrodes using potentiostatic electrochemical methods. The highest sensitivity in the determination of MTs was observed with a galvanostatic method such as derivative potentiometric stripping analysis (CPSA) which produces the peak H. The coupling of CPSA with the adsorptive transfer stripping technique (AdTS) allows the determination of MTs at a femtomole level in a low amount of sample (5 ěl). Our recent results obtained by AdTS + CPSA show that detection limits for particular MTs can be improved by adding [Co(NH3)6] Cl3 to the sample analyzed. This is probably due to the formation of a complex between [Co(NH3)6] Cl3 and MT. We think that the peak H can be used in physiological studies of metal metabolism.
Anglicky
Metallothioneins (MTs) belong to a group of oligo- and polypeptides which play an important role in the metabolism of metals in animals, plants and microorganisms. The metabolic function of MTs consists not only in detoxication of an organism but also in the homeostasis of essential metals. This paper reports a novel approach to electroanalysis of MTs. The electrochemical determination of MTs is based on catalytic processes which proceed at very negative potentials on mercury electrodes (from -1.7 V to -1.9 V vs. Ag/AgCl/KCl). These processes accompanied by evolution of hydrogen from supporting electrolyte components, include the Heyrovsky presodium wave and/or Brdicka reaction. It was found that SH groups present in MTs are responsible for catalytic processes. The catalytic signal of a MT at nanomolar concentrations can be detected on mercury electrodes using potentiostatic electrochemical methods. The highest sensitivity in the determination of MTs was observed with a galvanostatic method such as derivative potentiometric stripping analysis (CPSA) which produces the peak H. The coupling of CPSA with the adsorptive transfer stripping technique (AdTS) allows the determination of MTs at a femtomole level in a low amount of sample (5 ěl). Our recent results obtained by AdTS + CPSA show that detection limits for particular MTs can be improved by adding [Co(NH3)6] Cl3 to the sample analyzed. This is probably due to the formation of a complex between [Co(NH3)6] Cl3 and MT. We think that the peak H can be used in physiological studies of metal metabolism.
Návaznosti
| GA203/02/0422, projekt VaV |
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| IAA1163201, projekt VaV |
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