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@inproceedings{567329,
author = {Vacek, Jan and Trnková, Libuše and Jelen, František and Kizek, René},
address = {Thessaloniki, Greece},
booktitle = {Book of Abstracts - 55th Annual Meeting of the ISE},
edition = {1},
keywords = {metallothioneines; phytochelatins; glutathione; mercury electrode;catalytic reactions; Brdicka reaction;peak H},
language = {eng},
location = {Thessaloniki, Greece},
pages = {431 - 431},
publisher = {International Society of Electrochemistry},
title = {Electrochemical determination of cysteine-containing biomolecules by means of catalytic reactions on a mercury electrode},
year = {2004}
}
TY - JOUR
ID - 567329
AU - Vacek, Jan - Trnková, Libuše - Jelen, František - Kizek, René
PY - 2004
TI - Electrochemical determination of cysteine-containing biomolecules by means of catalytic reactions on a mercury electrode
PB - International Society of Electrochemistry
CY - Thessaloniki, Greece
KW - metallothioneines
KW - phytochelatins
KW - glutathione
KW - mercury electrode;catalytic reactions
KW - Brdicka reaction;peak H
N2 - The group cysteine-containing peptides (metallothioneines, phytochelatins, and glutathione) plays an important role in active homeostasis and detoxification of metals in animal, plant, and/or microorganism cells. In this study we describe a novel approach to electroanalysis of cys-containing peptides and proteins. This approach is based on catalytic processes (presodium wave, peak H, Brdicka current) proceeding at negative potentials (ca. 1.8 V, Ag/AgCl/3M KCl) on the hanging mercury drop electrode (HMDE). A presodium wave is current-potential response accompanied with catalytic hydrogen evolution from aqueous solutions in the presence of proteins. It was found that the sulfhydryl(SH) groups in proteins are responsible for presodium catalytic processes. The highest sensitivity in the determination was observed with derivative chronopotentiometric stripping analysis (CPSA), which monitored the catalytic signal known as peak H. The determination of the low content of Cys-peptides was successfully performed in a cobalt(III)-containing solution with ammonia buffer by differential pulse voltammetry (DPV). The CPSA (peak H) and DPV (Brdicka reaction) can be used in connection with the adsorptive transfer stripping (AdTS) technique, which is capable of determining nano/femtomole levels of metallothioneine, phytochelatin and glutathione in mL of the sample.
ER -
VACEK, Jan, Libuše TRNKOVÁ, František JELEN and René KIZEK. Electrochemical determination of cysteine-containing biomolecules by means of catalytic reactions on a mercury electrode. In \textit{Book of Abstracts - 55th Annual Meeting of the ISE}. 1st ed. Thessaloniki, Greece: International Society of Electrochemistry, 2004, p.~431 - 431.
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