The effect of nalidixic acid on growth and reproductive events
in nucleocytosolic and chloroplast compartments in the alga
Scenedesmus quadricauda

J 2004

The effect of nalidixic acid on growth and reproductive events in nucleocytosolic and chloroplast compartments in the alga Scenedesmus quadricauda

ZACHLEDER, Vilém, Shigeki KAWANO, Vladislav CEPÁK a Tsuneoshi KUROIWA

Základní údaje

Originální název

The effect of nalidixic acid on growth and reproductive events in nucleocytosolic and chloroplast compartments in the alga Scenedesmus quadricauda

Název česky

Vliv nalidixové kyseliny na růst a reprodukční děje v nukleocytoplasmě a chloroplastu u řasy Scenedesmus quadricauda

Autoři

ZACHLEDER, Vilém (203 Česká republika, garant), Shigeki KAWANO (392 Japonsko), Vladislav CEPÁK (203 Česká republika) a Tsuneoshi KUROIWA (392 Japonsko)

Vydání

Folia Microbiologica, Praha, Mikrobiologický ústav AV ČR, 2004, 0015-5632

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.034

Kód RIV

RIV/00216224:14330/04:00012034

Organizační jednotka

Fakulta informatiky

UT WoS

000224416700011

Klíčová slova anglicky

cell cycle; regulation; alga; Scenedesmus

Štítky

alga, cell cycle, regulation, Scenedesmus

Změněno: 2. 6. 2005 14:29, RNDr. JUDr. Vladimír Šmíd, CSc.

Anotace

ORIG CZ

V originále

The courses of rRNA accumulation, DNA replication, and nuclear division were followed both in the chloroplast and the nucleocytosolic compartments during the cell cycle in synchronized populations of the chlorococcal alga Scenedesmus quadricauda. Control and nalidixic acid treated cultures were compared. Nalidixic acid (150 mg/L) was added either at the beginning of the cell cycle or consecutively during the cell cycle to subcultures transferred into the dark. If the inhibitor was applied at the beginning of the cell cycle, chloroplast DNA did not replicate and nucleoids did not divide. Chloroplast division, however, was coordinated in a timely fashion with cytokinesis even under conditions of blocked chloroplast DNA replication. While the growth rate was slowed down, the courses of reproductive processes in the nucleocytosolic compartment were not affected and their timing and the number of rounds were coordinated with growth rate as in the control culture. The rate of cytosolic rRNA synthesis was slowed but no apparent effect was seen on the amount of rRNA that accumulated during the cell cycle. In contrast, lower levels of chloroplast rRNA were found at the end of the cell cycle compared with the control culture. Experiments in which cells were transferred to the dark during the cell cycle showed that the inhibitor affected none of the reproductive events in the nucleocytosolic compartment. In the chloroplast compartment, DNA replication was inhibited in inhibitor treated cultures, but was unaffected in controls. The chloroplast nucleoids themselves divided even in the presence of inhibitor, reducing their DNA content to a level which corresponded to that in freshly formed control daughter cells.

Česky

viz angl. verze

Návaznosti

GA204/02/1438, projekt VaV

Název: Změny v aktivitě komplexů cyklin dependentních kinas a ve fosforylaci jejich cílových bílkovin ve funkčním vztahu k dějům buněčného cyklu řas

Citovat

ZACHLEDER, Vilém, Shigeki KAWANO, Vladislav CEPÁK a Tsuneoshi KUROIWA. The effect of nalidixic acid on growth and reproductive events in nucleocytosolic and chloroplast compartments in the alga Scenedesmus quadricauda. Folia Microbiologica. Praha: Mikrobiologický ústav AV ČR, 2004, roč. 49, č. 4, s. 441-451. ISSN 0015-5632.

@article{568594,
   author = {Zachleder, Vilém and Kawano, Shigeki and Cepák, Vladislav and Kuroiwa, Tsuneoshi},
   article_location = {Praha},
   article_number = {4},
   keywords = {cell cycle; regulation; alga; Scenedesmus},
   language = {eng},
   issn = {0015-5632},
   journal = {Folia Microbiologica},
   title = {The effect of nalidixic acid on growth and reproductive events in nucleocytosolic and chloroplast compartments in the alga Scenedesmus quadricauda},
   volume = {49},
   year = {2004}
}

TY  - JOUR
ID  - 568594
AU  - Zachleder, Vilém - Kawano, Shigeki - Cepák, Vladislav - Kuroiwa, Tsuneoshi
PY  - 2004
TI  - The effect of nalidixic acid on growth and reproductive events in nucleocytosolic and chloroplast compartments in the alga Scenedesmus quadricauda
JF  - Folia Microbiologica
VL  - 49
IS  - 4
SP  - 441-451
EP  - 441-451
PB  - Mikrobiologický ústav AV ČR
SN  - 00155632
KW  - cell cycle
KW  - regulation
KW  - alga
KW  - Scenedesmus
N2  - The courses of rRNA accumulation, DNA replication, and nuclear division were followed both in the chloroplast and the nucleocytosolic compartments during the cell cycle in synchronized populations of the chlorococcal alga Scenedesmus quadricauda. Control and nalidixic acid treated cultures were compared. Nalidixic acid (150 mg/L) was added either at the beginning of the cell cycle or consecutively during the cell cycle to subcultures transferred into the dark. If the inhibitor was applied at the beginning of the cell cycle, chloroplast DNA did not replicate and nucleoids did not divide. Chloroplast division, however, was coordinated in a timely fashion with cytokinesis even under conditions of blocked chloroplast DNA replication. While the growth rate was slowed down, the courses of reproductive processes in the nucleocytosolic compartment were not affected and their timing and the number of rounds were coordinated with growth rate as in the control culture. The rate of cytosolic rRNA synthesis was slowed but no apparent effect was seen on the amount of rRNA that accumulated during the cell cycle. In contrast, lower levels of chloroplast rRNA were found at the end of the cell cycle compared with the control culture. Experiments in which cells were transferred to the dark during the cell cycle showed that the inhibitor affected none of the reproductive events in the nucleocytosolic compartment. In the chloroplast compartment, DNA replication was inhibited in inhibitor treated cultures, but was unaffected in controls. The chloroplast nucleoids themselves divided even in the presence of inhibitor, reducing their DNA content to a level which corresponded to that in freshly formed control daughter cells.
ER  -

ZACHLEDER, Vilém, Shigeki KAWANO, Vladislav CEPÁK a Tsuneoshi KUROIWA. The effect of nalidixic acid on growth and reproductive events in nucleocytosolic and chloroplast compartments in the alga Scenedesmus quadricauda. \textit{Folia Microbiologica}. Praha: Mikrobiologický ústav AV ČR, 2004, roč.~49, č.~4, s.~441-451. ISSN~0015-5632.

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