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@article{570037,
author = {Pohanka, Miroslav and Skládal, Petr},
article_location = {New York},
article_number = {3},
keywords = {piezoelectric immunosensor; tularemia; biowarfare agent},
language = {eng},
issn = {0003-2719},
journal = {Analytical Letters},
title = {Piezoelectric immunosensor for Francisella tularensis detection using immunoglobulin M in a limiting dilution},
volume = {38},
year = {2005}
}
TY - JOUR
ID - 570037
AU - Pohanka, Miroslav - Skládal, Petr
PY - 2005
TI - Piezoelectric immunosensor for Francisella tularensis detection using immunoglobulin M in a limiting dilution
JF - Analytical Letters
VL - 38
IS - 3
SP - 411-422
EP - 411-422
PB - Marcel Dekker
SN - 00032719
KW - piezoelectric immunosensor
KW - tularemia
KW - biowarfare agent
N2 - Francisella tularensis is a pathogenic bacterium causing the serious disease tularemia and is potentially considered as a bioweapon agent. The piezoelectric immunosensor for F. tularensis (Ft) assay was developed using the quartz crystal microbalance (QCM) with covalently immobilized Ft antigen. Samples containing the bacterium were preincubated with a highly diluted anti-Ft IgM, and the mixture was injected to the flow cell containing the immunosensor. IgM alone as well as Ft cells alone were not giving any response, and therefore, it is assumed that the sensor responded to the IgM(x)-Ft(y) complex formed during the preincubation step. The effects of pH, time of preincubation, and IgM concentration were tested. This rapid method allowed us to detect 5 (.) 10(6) Ft cells/mL. RSD of measurements was near 1%. The total time of one analysis was 35 min, and one sensor was used for more than 30 times without losing sensitivity.
ER -
POHANKA, Miroslav a Petr SKLÁDAL. Piezoelectric immunosensor for Francisella tularensis detection using immunoglobulin M in a limiting dilution. \textit{Analytical Letters}. New York: Marcel Dekker, 2005, roč.~38, č.~3, s.~411-422. ISSN~0003-2719.
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