Detailed Information on Publication Record
2005
Piezoelectric immunosensor for Francisella tularensis detection using immunoglobulin M in a limiting dilution
POHANKA, Miroslav and Petr SKLÁDALBasic information
Original name
Piezoelectric immunosensor for Francisella tularensis detection using immunoglobulin M in a limiting dilution
Name in Czech
Piezoelektrický imunosensor pro detekci Franciselly tularensis použitím imunoglobulinu M v limitním ředění
Authors
POHANKA, Miroslav (203 Czech Republic) and Petr SKLÁDAL (203 Czech Republic, guarantor)
Edition
Analytical Letters, New York, Marcel Dekker, 2005, 0003-2719
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 1.036
RIV identification code
RIV/00216224:14310/05:00013671
Organization unit
Faculty of Science
UT WoS
000227702200006
Keywords in English
piezoelectric immunosensor; tularemia; biowarfare agent
Změněno: 7/6/2005 16:07, prof. RNDr. Petr Skládal, CSc.
V originále
Francisella tularensis is a pathogenic bacterium causing the serious disease tularemia and is potentially considered as a bioweapon agent. The piezoelectric immunosensor for F. tularensis (Ft) assay was developed using the quartz crystal microbalance (QCM) with covalently immobilized Ft antigen. Samples containing the bacterium were preincubated with a highly diluted anti-Ft IgM, and the mixture was injected to the flow cell containing the immunosensor. IgM alone as well as Ft cells alone were not giving any response, and therefore, it is assumed that the sensor responded to the IgM(x)-Ft(y) complex formed during the preincubation step. The effects of pH, time of preincubation, and IgM concentration were tested. This rapid method allowed us to detect 5 (.) 10(6) Ft cells/mL. RSD of measurements was near 1%. The total time of one analysis was 35 min, and one sensor was used for more than 30 times without losing sensitivity.
In Czech
Byl vyvinut piezoelektrický imunosensor pro detekci patogenního mikroorganismu Francisella tularensis, působícího onemocnění tularemii. Analyzovaný vzorek se smíchá s velmi malým množstvím specifického IgM a vytvořený imunokomplex je změřen piezosensorem modifikovaným tularemickým antigenem. Doba stanovení je 35 minut, mez detekce 5x10^6 bakterií.
Links
| MSM0021622413, plan (intention) |
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