J 2005

Quartz crystal biosensor for detection of sugars and glycated hemoglobin

PŘIBYL, Jan and Petr SKLÁDAL

Basic information

Original name

Quartz crystal biosensor for detection of sugars and glycated hemoglobin

Name in Czech

Biosensor pro detekci sacharidů a glykovaného hemoglobinu na bázi křemenných krystalů

Authors

PŘIBYL, Jan (203 Czech Republic) and Petr SKLÁDAL (203 Czech Republic, guarantor)

Edition

Analytica Chimica Acta, Amsterdam, Elsevier Science Publishers, 2005, 0003-2670

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.760

RIV identification code

RIV/00216224:14310/05:00013672

Organization unit

Faculty of Science

UT WoS

000227102800011

Keywords in English

quartz crystal microbalance; m-aminophenylboronic acid; saccharides; glycated hemoglobin; kinetic analysis

Tags

Změněno: 7/6/2005 16:07, prof. RNDr. Petr Skládal, CSc.

Abstract

ORIG CZ

V originále

3-Aminophenylboronic acid (APBA) was used for construction of affinity mass sensors for determination of saccharides and glycated hemoglobin using complexation reaction with diol groups. Two approaches were tested for the bioligand layer fabrication-incorporation of APBA inside a thicker matrix and immobilization as a self-assembled monolayer, respectively. The direct affinity sensor with APBA embeded in the structure of glutaraldehyde-crosslinked bovine serum albumine provided linear response to mono- and dissacharides in the range from 0.1 to 15.0 mg/ml. This biosensor was also used for kinetic analysis of the interaction of boronic acid with diols; the values of association and dissociation constants were determined. The sensors with a monolayer of boronic groups were better suited for binding of glycated hemoglobin, probably due to improved steric access to the ligand.

In Czech

3-Aminofenylboritá kyselina (APBA) byla použita jako ligand imobilizovaný na povrchu piezoelektrického afinitního sensoru, který sloužil pro stanovení sacharidů (glukosa, fruktosa, sorbitol, aj.) a glykovaného hemoglobinu (marker u diabetiků). Byly porovnány různé typy imobilizačních postupů, poskytujících buď monovrstvu nebo matrix s navázanou APBA.

Links

MSM0021622413, plan (intention)
Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment