Amperometric screen-printed biosensor arrays with
co-immobilised oxidoreductases and cholinesterases

J 2005

Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases

SOLNÁ, Renáta, Eva DOCK, Alexander CHRISTENSON, Margrethe WINTER-NIELSEN, Christine CARLSSON et. al.

Základní údaje

Originální název

Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases

Název česky

Amperometrické sítotisková biosensorová pole s koimobilisovanými oxidoreduktasami a cholinesterasami

Autoři

SOLNÁ, Renáta (203 Česká republika), Eva DOCK (752 Švédsko), Alexander CHRISTENSON (752 Švédsko), Margrethe WINTER-NIELSEN (208 Dánsko), Christine CARLSSON (752 Švédsko), Jenny EMNÉUS (752 Švédsko), Tautgirdas RUZGAS (752 Švédsko) a Petr SKLÁDAL (203 Česká republika, garant)

Vydání

Analytica Chimica Acta, Amsterdam, Elsevier Science Publishers, 2005, 0003-2670

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.760

Kód RIV

RIV/00216224:14310/05:00013673

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000226455600002

Klíčová slova anglicky

amperometric multienzyme sensor; steady state system; cholinesterase; oxidoreductase; phenoks; pesticides; wastewater; multivariate-analysis

Štítky

amperometric multienzyme sensor, Cholinesterase, multivariate-analysis, oxidoreductase, pesticides, phenoks, steady state system, wastewater

Změněno: 7. 6. 2005 16:04, prof. RNDr. Petr Skládal, CSc.

Anotace

ORIG CZ

V originále

Amperometric screen-printed biosensor arrays for detection of pesticides (organophosphates and carbamates) and phenols have been developed. Cholinesterases (AChE and BChE), tyrosinase (TYR), peroxidases (SBP. soybean and HRP. horseradish) and cellobiose dehydrogenase (CDH) were combined on the same array consisting of one Ag/AgCl reference electrode surrounded by eight radially distributed working electrodes of either carbon or platinum. Mainly cross-linking with glutaraldehyde was employed for enzyme immobilisation. The substrates for the enzymes were acetylthiocholine for cholinesterases (ChEs), cellobiose for CDH and hydrogen peroxide for peroxidases. Hydrogen peroxide was generated in the presence of glucose by co-immobilised glucose oxidase (GOx). All measurements were performed in an electrochemical steady state system specially constructed for eight channel screen-printed electrode arrays. The achieved relative standard deviation values calculated for different enzyme substrates (10 measurements) were typically below 7% and one assay was completed within less than 10 min. The detection limits for pesticides and phenols were in the nanomolar and micromolar ranges, respectively. The developed biosensor array was evaluated on wastewater samples. To simplify interpretation of results. the measured data were created with multivariate analysis-principal component analysis (PCA).

Česky

Amperometrické sítotiskové multielektrodové biosensory byly připraveny použitím cholinesteras (AChE a BChE), tyrosinasy, celobiosa dehydrogenasy a peroxidas (křenová a sojová) na jediném sensoru. Byly optimalisovány podmínky měření. Testované vzorky odpadních vod pak byly klasifikovány pomocí chemometrické multivariační analysy.

Návaznosti

MSM0021622413, záměr

Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím

Citovat

SOLNÁ, Renáta, Eva DOCK, Alexander CHRISTENSON, Margrethe WINTER-NIELSEN, Christine CARLSSON, Jenny EMNÉUS, Tautgirdas RUZGAS a Petr SKLÁDAL. Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases. Analytica Chimica Acta. Amsterdam: Elsevier Science Publishers, 2005, roč. 528, č. 1, s. 9-19. ISSN 0003-2670.

@article{570041,
   author = {Solná, Renáta and Dock, Eva and Christenson, Alexander and WinterandNielsen, Margrethe and Carlsson, Christine and Emnéus, Jenny and Ruzgas, Tautgirdas and Skládal, Petr},
   article_location = {Amsterdam},
   article_number = {1},
   keywords = {amperometric multienzyme sensor; steady state system; cholinesterase; oxidoreductase; phenoks; pesticides; wastewater; multivariate-analysis},
   language = {eng},
   issn = {0003-2670},
   journal = {Analytica Chimica Acta},
   title = {Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases},
   volume = {528},
   year = {2005}
}

TY  - JOUR
ID  - 570041
AU  - Solná, Renáta - Dock, Eva - Christenson, Alexander - Winter-Nielsen, Margrethe - Carlsson, Christine - Emnéus, Jenny - Ruzgas, Tautgirdas - Skládal, Petr
PY  - 2005
TI  - Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases
JF  - Analytica Chimica Acta
VL  - 528
IS  - 1
SP  - 9-19
EP  - 9-19
PB  - Elsevier Science Publishers
SN  - 00032670
KW  - amperometric multienzyme sensor
KW  - steady state system
KW  - cholinesterase
KW  - oxidoreductase
KW  - phenoks
KW  - pesticides
KW  - wastewater
KW  - multivariate-analysis
N2  - Amperometric screen-printed biosensor arrays for detection of pesticides (organophosphates and carbamates) and phenols have been developed. Cholinesterases (AChE and BChE), tyrosinase (TYR), peroxidases (SBP. soybean and HRP. horseradish) and cellobiose dehydrogenase (CDH) were combined on the same array consisting of one Ag/AgCl reference electrode surrounded by eight radially distributed working electrodes of either carbon or platinum. Mainly cross-linking with glutaraldehyde was employed for enzyme immobilisation. The substrates for the enzymes were acetylthiocholine for cholinesterases (ChEs), cellobiose for CDH and hydrogen peroxide for peroxidases. Hydrogen peroxide was generated in the presence of glucose by co-immobilised glucose oxidase (GOx). All measurements were performed in an electrochemical steady state system specially constructed for eight channel screen-printed electrode arrays. The achieved relative standard deviation values calculated for different enzyme substrates (10 measurements) were typically below 7% and one assay was completed within less than 10 min. The detection limits for pesticides and phenols were in the nanomolar and micromolar ranges, respectively. The developed biosensor array was evaluated on wastewater samples. To simplify interpretation of results. the measured data were created with multivariate analysis-principal component analysis (PCA).
ER  -

SOLNÁ, Renáta, Eva DOCK, Alexander CHRISTENSON, Margrethe WINTER-NIELSEN, Christine CARLSSON, Jenny EMNÉUS, Tautgirdas RUZGAS a Petr SKLÁDAL. Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier Science Publishers, 2005, roč.~528, č.~1, s.~9-19. ISSN~0003-2670.

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