HAVLIŠ, Jan, Tobias OELSCHLAEGEL, Martin SCHWICKART, Joao MATOS, Aliona BOGDANOVA, Alain CAMASSES, Andrej SHEVCHENKO a Wolfgang ZACHARIAE. The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1. Cell. United States of America: Cell Press, Cambridge, MA, USA, 2005, roč. 120, č. 6, s. 773-788, 15 s. ISSN 0092-8674. |
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@article{571157, author = {Havliš, Jan and Oelschlaegel, Tobias and Schwickart, Martin and Matos, Joao and Bogdanova, Aliona and Camasses, Alain and Shevchenko, Andrej and Zachariae, Wolfgang}, article_location = {United States of America}, article_number = {6}, keywords = {anaphase-promoting complex; chromosome segregation; fission yeast; centromeric cohesion; spore formation; protein family; kinase cdc5; cleavage; mitosis; proteolysis}, language = {eng}, issn = {0092-8674}, journal = {Cell}, title = {The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1}, volume = {120}, year = {2005} }
TY - JOUR ID - 571157 AU - Havliš, Jan - Oelschlaegel, Tobias - Schwickart, Martin - Matos, Joao - Bogdanova, Aliona - Camasses, Alain - Shevchenko, Andrej - Zachariae, Wolfgang PY - 2005 TI - The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1 JF - Cell VL - 120 IS - 6 SP - 773-788 EP - 773-788 PB - Cell Press, Cambridge, MA, USA SN - 00928674 KW - anaphase-promoting complex KW - chromosome segregation KW - fission yeast KW - centromeric cohesion KW - spore formation KW - protein family KW - kinase cdc5 KW - cleavage KW - mitosis KW - proteolysis N2 - Cohesion established between sister chromatids during pre-meiotic DNA replication mediates two rounds of chromosome segregation. The first division is preceded by an extended prophase wherein homologous chromosomes undergo recombination. The persistence of cohesion during prophase is essential for recombination and both meiotic divisions. Here we show that Mnd2, a subunit of the anaphase-promoting complex (APC/C) from budding yeast, is essential to prevent premature destruction of cohesion in meiosis. During S- and prophase, Mnd2 prevents activation of the APC/C by a melosis-specific activator called Ama1. In cells lacking Mnd2 the APC/C-Ama1 enzyme triggers degradation of Pds1, which causes premature sister chromatid separation due to unrestrained separase activity. In vitro, Mnd2 inhibits ubiquitination of Pds1 by APC/C-Ama1 but not by other APC/C holo-enzymes. We conclude that chromosome segregation in meiosis depends on the selective inhibition of a meiosis-specific form of the APC/C. ER -
HAVLIŠ, Jan, Tobias OELSCHLAEGEL, Martin SCHWICKART, Joao MATOS, Aliona BOGDANOVA, Alain CAMASSES, Andrej SHEVCHENKO a Wolfgang ZACHARIAE. The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1. \textit{Cell}. United States of America: Cell Press, Cambridge, MA, USA, 2005, roč.~120, č.~6, s.~773-788, 15 s. ISSN~0092-8674.
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