Detailed Information on Publication Record
2005
Cytoskeleton during the cell division cycle of the yeast Cryptococcus laurentii
DAVID, Marek, Miroslav GABRIEL and Marie KOPECKÁBasic information
Original name
Cytoskeleton during the cell division cycle of the yeast Cryptococcus laurentii
Authors
DAVID, Marek (203 Czech Republic, belonging to the institution), Miroslav GABRIEL (203 Czech Republic, guarantor, belonging to the institution) and Marie KOPECKÁ (203 Czech Republic, belonging to the institution)
Edition
XIII. Cytoskeletální klub, Hotel SKI, Nové Město na Moravě 27.-29.4.2005, 2005
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
30000 3. Medical and Health Sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14110/05:00012467
Organization unit
Faculty of Medicine
Keywords in English
basidiomycetous yeast; Cryptococcus laurentii; cytoskeleton; F-actin; microtubules
Změněno: 29/3/2011 14:07, Mgr. Marek David, Ph.D.
Abstract
V originále
The cytoskeleton was investigated in Cryptococcus laurentii. The actin cytoskeleton stained by rhodamine-phalloidin in encapsulated strain CCY 17-3-5 failed, but the actin patches were detected by application of monoclonal anti-actin antibody and secondary antibody SwAM-FITC. In acapsular strain C. laurentii 17-3-6, F-actin was stainable by rhodamine-phalloidin. The cortical actin patches were distributed under cell surface and actin cables extended to the bud. At cytokinesis, the actin cables were replaced with contractile ring. Microtubules were seen in both yeast strains. Cytoplasmatic microtubules during interphase arranged into a random network under the plasma membrane. Before mitosis, nucleus migrated from the mother cell to the bud, cytoplasmatic microtubules were disassembled, the spindle formed that allowed the nuclear division. Before the cytokinesis the cytoplasmatic microtubules reappeared. The dynamics of cytoskeleton in C. laurentii is similar to Cryptococcus neoformans (2).
Links
| GA310/03/1195, research and development project |
|