MATĚJKOVÁ, Petra, David ŠMAJS, SJ NORRIS a GM WEINSTOCK. Comparative genomics of pathogenic Treponema pallidum species. In Sborník příspěvků VII. Pracovní setkání biochemiků a molekulárních biologů. 2003. vyd. Brno: Masarykova univerzita, 2003. s. 3 - 4, 1 s. ISBN 80-210-3053-4.
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Základní údaje
Originální název Comparative genomics of pathogenic Treponema pallidum species
Název česky Komparativní genomika patogenních druhů Treponema pallidum
Název anglicky Comparative genomics of pathogenic Treponema pallidum species
Autoři MATĚJKOVÁ, Petra (203 Česká republika), David ŠMAJS (203 Česká republika, garant), SJ NORRIS (840 Spojené státy americké) a GM WEINSTOCK (840 Spojené státy americké).
Vydání 2003. vyd. Brno, Sborník příspěvků VII. Pracovní setkání biochemiků a molekulárních biologů, od s. 3 - 4, 1 s. 2003.
Nakladatel Masarykova univerzita
Další údaje
Originální jazyk čeština
Typ výsledku Stať ve sborníku
Obor Genetika a molekulární biologie
Stát vydavatele Česká republika
Utajení není předmětem státního či obchodního tajemství
Kód RIV RIV/00216224:14110/03:00012306
Organizační jednotka Lékařská fakulta
ISBN 80-210-3053-4
Klíčová slova anglicky comparative genomics; Treponema pallidum
Štítky comparative genomics, Treponema pallidum
Změnil Změnil: prof. MUDr. David Šmajs, Ph.D., učo 1116. Změněno: 31. 5. 2005 09:29.
Anotace
Spirochaetal genus Treponema includes several pathogenic spirochetes (e.g. Treponema pallidum subsp. pallidum is the causative agent of syphilis, T. pallidum subsp. pertenue causes yaws). Recent serological tests are negative in early stages of treponemal infection and cannot distinguish between syphilis and yaws. The complete genome sequence, construction of a microarray chip with all 1039 predicted ORF PCR products, together with the findings that there is a high degree of sequence homology among pathogenic treponemes, enables comparative genomic analyses based on DNA-microarray techniques. Identification of chromosomal sequences specific for these pathogens can be used for selective PCR diagnostics of treponemal diseases. DNA of Treponema pallidum subsp. pallidum Nichols strain was compared to DNA isolated from three different strains of T. pallidum subsp. pertenue (strain Gauthier, Samoan D, CDC-2). As a result of DNA microarray comparisons, 25 genes (13 with stronger and 6 with weaker signal in pertenue strains and 6 control genes with similar signal in both subspecies examined) were selected and sequenced. Altogether, 24083 nucleotides (2.12% of the genome) were sequenced in 3 pertenue strains and control Nichols. No region of extensive sequence heterogeneity was detected. However, 15 different single nucleotide polymorfisms (SNP) were identified: 3 SNPs in Gauthier strain, 14 in Samoan D and 15 SNPs in CDC-2. Ten (out of 15) SNPs cause amino acid changes. SNPs common for all pertenue strains as well as SNPs specific for each individual strain will allow to use these nucleotide polymorfisms to design sequence specific PCR diagnostics of these strains.
Anotace anglicky
Spirochaetal genus Treponema includes several pathogenic spirochetes (e.g. Treponema pallidum subsp. pallidum is the causative agent of syphilis, T. pallidum subsp. pertenue causes yaws). Recent serological tests are negative in early stages of treponemal infection and cannot distinguish between syphilis and yaws. The complete genome sequence, construction of a microarray chip with all 1039 predicted ORF PCR products, together with the findings that there is a high degree of sequence homology among pathogenic treponemes, enables comparative genomic analyses based on DNA-microarray techniques. Identification of chromosomal sequences specific for these pathogens can be used for selective PCR diagnostics of treponemal diseases. DNA of Treponema pallidum subsp. pallidum Nichols strain was compared to DNA isolated from three different strains of T. pallidum subsp. pertenue (strain Gauthier, Samoan D, CDC-2). As a result of DNA microarray comparisons, 25 genes (13 with stronger and 6 with weaker signal in pertenue strains and 6 control genes with similar signal in both subspecies examined) were selected and sequenced. Altogether, 24083 nucleotides (2.12% of the genome) were sequenced in 3 pertenue strains and control Nichols. No region of extensive sequence heterogeneity was detected. However, 15 different single nucleotide polymorfisms (SNP) were identified: 3 SNPs in Gauthier strain, 14 in Samoan D and 15 SNPs in CDC-2. Ten (out of 15) SNPs cause amino acid changes. SNPs common for all pertenue strains as well as SNPs specific for each individual strain will allow to use these nucleotide polymorfisms to design sequence specific PCR diagnostics of these strains.
Návaznosti
NI7351, projekt VaVNázev: Komparativní genomika patogenních spirochet rodu Treponema: cesta k sekvenčně specifické diagnostice
Investor: Ministerstvo zdravotnictví ČR, Infekční choroby, mikrobiologie, epidemiologie a imunologie
VytisknoutZobrazeno: 17. 2. 2020 07:29