2003
Transkriptom spirochety Treponema pallidum: úroveň genové exprese během experimentální infekce králíků
ŠMAJS, David, Petra MATĚJKOVÁ, SJ NORRIS a GM WEINSTOCKZákladní údaje
Originální název
Transkriptom spirochety Treponema pallidum: úroveň genové exprese během experimentální infekce králíků
Název česky
Transkriptom spirochety Treponema pallidum: úroveň genové exprese během experimentální infekce králíků
Název anglicky
Transkriptome of Treponema pallidum: gene expression levels during experimental rabbit infection
Autoři
ŠMAJS, David (203 Česká republika, garant), Petra MATĚJKOVÁ (203 Česká republika), SJ NORRIS (840 Spojené státy) a GM WEINSTOCK (840 Spojené státy)
Vydání
2003. vyd. Brno, Sborník abstraktů XII. konference mladých mikrobiologů Tomáškovy dny 2003, od s. 14 - 15, 1 s. 2003
Nakladatel
Lékařská fakulta MU
Další údaje
Jazyk
čeština
Typ výsledku
Stať ve sborníku
Obor
Genetika a molekulární biologie
Stát vydavatele
Estonsko
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14110/03:00012309
Organizační jednotka
Lékařská fakulta
Klíčová slova anglicky
transcriptome; treponema pallidum
Štítky
Změněno: 31. 5. 2005 10:31, prof. MUDr. David Šmajs, Ph.D.
V originále
DNA microarray technology was utilized to study gene expression by the syphilis spirochete Treponema pallidum subsp. pallidum (Nichols) isolated from rabbit testes 10 days post infection. Microarrays containing all 1039 annotated ORFs of Treponema pallidum subspecies pallidum (Nichols) were printed on glass slides. For 1034 ORFs (out of 1039), signals higher than the threshold (average of negative control spots + 3 SDs) were detected for both RNA and DNA probes. The most highly transcribed genes were found to correlate with the most conspicuous spots identified by two dimensional gel electrophoresis, indicating that the transcript levels generally corresponded to the relative protein concentrations. Genes with high transcript concentrations included those encoding flagellar filament and cytoplasmic filament proteins, prominent lipoproteins and membrane proteins, chaperonins, proteins involved in red-ox balance, chemotaxis regulatory proteins, a V-ATPase operon, and certain metabolic enzymes such as glycolytic pathway enzymes. Independent quantitation of the expression of 84 T. pallidum genes using real-time RT-PCR approach yielded a high degree of correlation (r = 0.94). Characterization of the T. pallidum transcriptome during experimental infection provides further insight into the importance of gene expression levels in the survival and pathogenesis of this bacterium in the mammalian host.
Anglicky
DNA microarray technology was utilized to study gene expression by the syphilis spirochete Treponema pallidum subsp. pallidum (Nichols) isolated from rabbit testes 10 days post infection. Microarrays containing all 1039 annotated ORFs of Treponema pallidum subspecies pallidum (Nichols) were printed on glass slides. For 1034 ORFs (out of 1039), signals higher than the threshold (average of negative control spots + 3 SDs) were detected for both RNA and DNA probes. The most highly transcribed genes were found to correlate with the most conspicuous spots identified by two dimensional gel electrophoresis, indicating that the transcript levels generally corresponded to the relative protein concentrations. Genes with high transcript concentrations included those encoding flagellar filament and cytoplasmic filament proteins, prominent lipoproteins and membrane proteins, chaperonins, proteins involved in red-ox balance, chemotaxis regulatory proteins, a V-ATPase operon, and certain metabolic enzymes such as glycolytic pathway enzymes. Independent quantitation of the expression of 84 T. pallidum genes using real-time RT-PCR approach yielded a high degree of correlation (r = 0.94). Characterization of the T. pallidum transcriptome during experimental infection provides further insight into the importance of gene expression levels in the survival and pathogenesis of this bacterium in the mammalian host.
Návaznosti
| NI7351, projekt VaV |
|