ŠMAJS, David, Petra MATĚJKOVÁ, SJ NORRIS and GM WEINSTOCK. Transcriptome of Treponema pallidum: gene expression profile during experimental rabbit infection. CHEMICKÉ LISTY, Praha: Česká společnost chemická, 2003, vol. 2003, No 97, p. 311. ISSN 0009-2770.
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Basic information
Original name Transcriptome of Treponema pallidum: gene expression profile during experimental rabbit infection
Name in Czech Transcriptom Treponema pallidum: expresní profilování během experimentální infekce králíků
Authors ŠMAJS, David (203 Czech Republic, guarantor), Petra MATĚJKOVÁ (203 Czech Republic), SJ NORRIS (840 United States of America) and GM WEINSTOCK (840 United States of America).
Edition CHEMICKÉ LISTY, Praha, Česká společnost chemická, 2003, 0009-2770.
Other information
Original language English
Type of outcome article in a journal
Field of Study Genetics and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.345
RIV identification code RIV/00216224:14110/03:00012310
Organization unit Faculty of Medicine
UT WoS 000227191600037
Keywords in English transcriptome; Treponema pallidum; gene expression profiling
Tags gene expression profiling, transcriptome, Treponema pallidum
Changed by Changed by: prof. MUDr. David Šmajs, Ph.D., učo 1116. Changed: 31/5/2005 10:03.
Abstract
DNA microarray technology was utilized to study gene expression by the syphilis spirochete Treponema pallidum subsp. pallidum (Nichols) during infection of rabbits. Microarrays containing all 1039 annotated ORFs of Treponema pallidum subspecies pallidum (Nichols) were printed on glass slides. For 1034 ORFs (out of 1039), signals higher than the threshold (average of negative control spots + 3 SDs) were detected for both RNA and DNA probes. Total RNA from T. pallidum isolated from rabbit testes 10 days post infection was labeled and standardized by cohybridization of the same arrays with treponemal chromosomal DNA labeled with a different fluorescent marker. This internal standardization technique proved to be highly reproducible and to decrease the impact of variables such as host nucleic acid contamination or variable target DNA lengths. The most highly transcribed genes were found to correlate with the most conspicuous spots identified by two dimensional gel electrophoresis, indicating that the transcript levels generally corresponded to the relative protein concentrations. Genes with high transcript concentrations included those encoding flagellar filament and cytoplasmic filament proteins, prominent lipoproteins and membrane proteins, chaperonins, proteins involved in red-ox balance, chemotaxis regulatory proteins, a V-ATPase operon, and certain metabolic enzymes such as glycolytic pathway enzymes. Independent quantitation of the expression of 84 T. pallidum genes using real-time RT-PCR approach yielded a high degree of correlation (r = 0.94). Characterization of the T. pallidum transcriptome during experimental infection provides further insight into the importance of gene expression levels in the survival and pathogenesis of this bacterium in the mammalian host.
Abstract (in Czech)
Anotace česky: Byl vyšetřen transkriptom Treponema pallidum během experimentální infekce králíků pomocí DNA microarray čipů obsahujících všech 1039 anotovaných ORF. Geny Treponema pallidum byly rozděleny do skupin podle úrovně transkripce.
Links
NI7351, research and development projectName: Komparativní genomika patogenních spirochet rodu Treponema: cesta k sekvenčně specifické diagnostice
Investor: Ministry of Health of the CR, Infectious Diseases, Microbiology, Epidemiology and Immunology
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