Transcriptome of Treponema pallidum: gene expression profile
during experimental rabbit infection

J 2003

Transcriptome of Treponema pallidum: gene expression profile during experimental rabbit infection

ŠMAJS, David, Petra MATĚJKOVÁ, SJ NORRIS and GM WEINSTOCK

Basic information

Original name

Transcriptome of Treponema pallidum: gene expression profile during experimental rabbit infection

Name in Czech

Transcriptom Treponema pallidum: expresní profilování během experimentální infekce králíků

Authors

ŠMAJS, David (203 Czech Republic, guarantor), Petra MATĚJKOVÁ (203 Czech Republic), SJ NORRIS (840 United States of America) and GM WEINSTOCK (840 United States of America)

Edition

CHEMICKÉ LISTY, Praha, Česká společnost chemická, 2003, 0009-2770

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.345

RIV identification code

RIV/00216224:14110/03:00012310

Organization unit

Faculty of Medicine

UT WoS

000227191600037

Keywords in English

transcriptome; Treponema pallidum; gene expression profiling

Abstract

ORIG CZ

V originále

DNA microarray technology was utilized to study gene expression by the syphilis spirochete Treponema pallidum subsp. pallidum (Nichols) during infection of rabbits. Microarrays containing all 1039 annotated ORFs of Treponema pallidum subspecies pallidum (Nichols) were printed on glass slides. For 1034 ORFs (out of 1039), signals higher than the threshold (average of negative control spots + 3 SDs) were detected for both RNA and DNA probes. Total RNA from T. pallidum isolated from rabbit testes 10 days post infection was labeled and standardized by cohybridization of the same arrays with treponemal chromosomal DNA labeled with a different fluorescent marker. This internal standardization technique proved to be highly reproducible and to decrease the impact of variables such as host nucleic acid contamination or variable target DNA lengths. The most highly transcribed genes were found to correlate with the most conspicuous spots identified by two dimensional gel electrophoresis, indicating that the transcript levels generally corresponded to the relative protein concentrations. Genes with high transcript concentrations included those encoding flagellar filament and cytoplasmic filament proteins, prominent lipoproteins and membrane proteins, chaperonins, proteins involved in red-ox balance, chemotaxis regulatory proteins, a V-ATPase operon, and certain metabolic enzymes such as glycolytic pathway enzymes. Independent quantitation of the expression of 84 T. pallidum genes using real-time RT-PCR approach yielded a high degree of correlation (r = 0.94). Characterization of the T. pallidum transcriptome during experimental infection provides further insight into the importance of gene expression levels in the survival and pathogenesis of this bacterium in the mammalian host.

In Czech

Anotace česky: Byl vyšetřen transkriptom Treponema pallidum během experimentální infekce králíků pomocí DNA microarray čipů obsahujících všech 1039 anotovaných ORF. Geny Treponema pallidum byly rozděleny do skupin podle úrovně transkripce.

Links

NI7351, research and development project

Name: Komparativní genomika patogenních spirochet rodu Treponema: cesta k sekvenčně specifické diagnostice

Investor: Ministry of Health of the CR

Citovat

ŠMAJS, David, Petra MATĚJKOVÁ, SJ NORRIS and GM WEINSTOCK. Transcriptome of Treponema pallidum: gene expression profile during experimental rabbit infection. CHEMICKÉ LISTY. Praha: Česká společnost chemická, 2003, vol. 2003, No 97, p. 311. ISSN 0009-2770.

@article{571747,
   author = {Šmajs, David and Matějková, Petra and Norris, SJ and Weinstock, GM},
   article_location = {Praha},
   article_number = {97},
   keywords = {transcriptome; Treponema pallidum; gene expression profiling},
   language = {eng},
   issn = {0009-2770},
   journal = {CHEMICKÉ LISTY},
   title = {Transcriptome of Treponema pallidum: gene expression profile during experimental rabbit infection},
   volume = {2003},
   year = {2003}
}

TY  - JOUR
ID  - 571747
AU  - Šmajs, David - Matějková, Petra - Norris, SJ - Weinstock, GM
PY  - 2003
TI  - Transcriptome of Treponema pallidum: gene expression profile during experimental rabbit infection
JF  - CHEMICKÉ LISTY
VL  - 2003
IS  - 97
SP  - 311
EP  - 311
PB  - Česká společnost chemická
SN  - 00092770
KW  - transcriptome
KW  - Treponema pallidum
KW  - gene expression profiling
N2  - DNA microarray technology was utilized to study gene expression by the syphilis spirochete Treponema pallidum subsp. pallidum (Nichols) during infection of rabbits. Microarrays containing all 1039 annotated ORFs of Treponema pallidum subspecies pallidum (Nichols) were printed on glass slides. For 1034 ORFs (out of 1039), signals higher than the threshold (average of negative control spots + 3 SDs) were detected for both RNA and DNA probes. Total RNA from T. pallidum isolated from rabbit testes 10 days post infection was labeled and standardized by cohybridization of the same arrays with treponemal chromosomal DNA labeled with a different fluorescent marker. This internal standardization technique proved to be highly reproducible and to decrease the impact of variables such as host nucleic acid contamination or variable target DNA lengths. The most highly transcribed genes were found to correlate with the most conspicuous spots identified by two dimensional gel electrophoresis, indicating that the transcript levels generally corresponded to the relative protein concentrations. Genes with high transcript concentrations included those encoding flagellar filament and cytoplasmic filament proteins, prominent lipoproteins and membrane proteins, chaperonins, proteins involved in red-ox balance, chemotaxis regulatory proteins, a V-ATPase operon, and certain metabolic enzymes such as glycolytic pathway enzymes. Independent quantitation of the expression of 84 T. pallidum genes using real-time RT-PCR approach yielded a high degree of correlation (r = 0.94). Characterization of the T. pallidum transcriptome during experimental infection provides further insight into the importance of gene expression levels in the survival and pathogenesis of this bacterium in the mammalian host.
ER  -

ŠMAJS, David, Petra MATĚJKOVÁ, SJ NORRIS and GM WEINSTOCK. Transcriptome of Treponema pallidum: gene expression profile during experimental rabbit infection. \textit{CHEMICKÉ LISTY}. Praha: Česká společnost chemická, 2003, vol.~2003, No~97, p.~311. ISSN~0009-2770.

Detailed Information on Publication Record