2003
Komparativní genomika patogenních poddruhů treponema pallidum
MATĚJKOVÁ, Petra, David ŠMAJS, SJ NORRIS a GM WEINSTOCKZákladní údaje
Originální název
Komparativní genomika patogenních poddruhů treponema pallidum
Název česky
Komparativní genomika patogenních poddruhů Treponema pallidum
Název anglicky
Comparative genomics of pathogenic subspecies of Treponema pallidum
Autoři
MATĚJKOVÁ, Petra (203 Česká republika), David ŠMAJS (203 Česká republika, garant), SJ NORRIS (840 Spojené státy) a GM WEINSTOCK (840 Spojené státy)
Vydání
2003. vyd. Brno, Sborník abstraktů XII. konference mladých mikrobiologů Tomáškovy dny 2003, s. 14-14, 2003
Nakladatel
Lékařská fakulta MU
Další údaje
Jazyk
čeština
Typ výsledku
Stať ve sborníku
Obor
Genetika a molekulární biologie
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14110/03:00012311
Organizační jednotka
Lékařská fakulta
Klíčová slova anglicky
comparative genomics; Treponema pallidum
Změněno: 31. 5. 2005 10:35, prof. MUDr. David Šmajs, Ph.D.
V originále
As a result of DNA microarray comparisons, 25 genes (13 with stronger and 6 with weaker signal in pertenue strains and 6 control genes with similar signal in both subspecies examined) were selected and sequenced. Altogether, 24083 nucleotides (2.12% of the genome) were sequenced in 3 pertenue strains and control Nichols. No region of extensive sequence heterogeneity was detected. However, 15 different single nucleotide polymorfisms (SNP) were identified: 3 SNPs in Gauthier strain, 14 in Samoan D and 15 SNPs in CDC-2. Ten (out of 15) SNPs cause amino acid changes. SNPs common for all pertenue strains as well as SNPs specific for each individual strain will allow to use these nucleotide polymorfisms to design sequence specific PCR diagnostics of these strains.
Anglicky
As a result of DNA microarray comparisons, 25 genes (13 with stronger and 6 with weaker signal in pertenue strains and 6 control genes with similar signal in both subspecies examined) were selected and sequenced. Altogether, 24083 nucleotides (2.12% of the genome) were sequenced in 3 pertenue strains and control Nichols. No region of extensive sequence heterogeneity was detected. However, 15 different single nucleotide polymorfisms (SNP) were identified: 3 SNPs in Gauthier strain, 14 in Samoan D and 15 SNPs in CDC-2. Ten (out of 15) SNPs cause amino acid changes. SNPs common for all pertenue strains as well as SNPs specific for each individual strain will allow to use these nucleotide polymorfisms to design sequence specific PCR diagnostics of these strains.
Návaznosti
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