Detailed Information on Publication Record
2003
Comparative genomics of pathogenic Treponema pallidum subspecies
MATĚJKOVÁ, Petra, David ŠMAJS, SJ NORRIS and GM WEINSTOCKBasic information
Original name
Comparative genomics of pathogenic Treponema pallidum subspecies
Name in Czech
Komparativní genomika patogenních poddruhů Treponema pallidum
Authors
MATĚJKOVÁ, Petra (203 Czech Republic), David ŠMAJS (203 Czech Republic, guarantor), SJ NORRIS (840 United States of America) and GM WEINSTOCK (840 United States of America)
Edition
CHEMICKÉ LISTY, Praha, Česká společnost chemická, 2003, 0009-2770
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 0.345
RIV identification code
RIV/00216224:14110/03:00012312
Organization unit
Faculty of Medicine
Keywords in English
comparative genomics; Treponema pallidum
Změněno: 31/5/2005 10:12, prof. MUDr. David Šmajs, Ph.D.
V originále
DNA of Treponema pallidum subsp. pallidum Nichols strain was compared to DNA isolated from three different strains of T. pallidum subsp. pertenue (strain Gauthier, Samoan D, CDC-2). As a result of DNA microarray comparisons, 25 genes (13 with stronger and 6 with weaker signal in pertenue strains and 6 control genes with similar signal in both subspecies examined) were selected and sequenced. Altogether, 24083 nucleotides (2.12% of the genome) were sequenced in 3 pertenue strains and control Nichols. No region of extensive sequence heterogeneity was detected. However, 15 different single nucleotide polymorfisms (SNP) were identified: 3 SNPs in Gauthier strain, 14 in Samoan D and 15 SNPs in CDC-2. Ten (out of 15) SNPs cause amino acid changes. SNPs common for all pertenue strains as well as SNPs specific for each individual strain will allow to use these nucleotide polymorfisms to design sequence specific PCR diagnostics of these strains.
In Czech
Byly vyšetřeny kmeny Treponema pallidum subsp. pallidum (původce syfilis) a T. pallidum subsp. pertenue (původce yaws) pomocí DNA-microarray techniky a genomového sekvenování. Zjištěné rozdíly byly diskutovány v kontextu současných poznatků.
Links
| NI7351, research and development project |
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