Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1-
and 2-Bond Residual Dipolar Couplings

D 2004

Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1- and 2-Bond Residual Dipolar Couplings

NOVÁK, Petr, Lukáš ŽÍDEK, Petr PADRTA a Vladimír SKLENÁŘ

Základní údaje

Originální název

Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1- and 2-Bond Residual Dipolar Couplings

Název česky

Rafinace struktury Trypanosoma U6 RNA s využitím jedno- a dvouvazebných zbytkových dipolárních interakcí

Autoři

NOVÁK, Petr (203 Česká republika), Lukáš ŽÍDEK (203 Česká republika), Petr PADRTA (203 Česká republika) a Vladimír SKLENÁŘ (203 Česká republika, garant)

Vydání

Brno, 19th NMR Valtice, od s. 30-30, 1 s. 2004

Nakladatel

Masarykova universita v Brně

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/04:00011133

Organizační jednotka

Přírodovědecká fakulta

ISBN

80-210-3352-5

Klíčová slova anglicky

NMR; Residual dipolar couplings; RNA; structure;

Štítky

NMR, residual dipolar couplings, RNA, structure

Změněno: 31. 5. 2005 13:42, Mgr. Petr Novák, Ph.D.

Anotace

ORIG CZ

V originále

The spliceosome is a complex of proteins and small nuclear RNAs (snRNAs) responsible for the removal of introns from pre-mRNA in eukaryotes. U6 snRNA has been demonstrated to be a vital element in splicing and its sequence is the most conserved of all snRNAs[1]. U6 snRNA from trypanosoma was our study. Determination of the solution structure of nucleic acid fragments is significantly improved if residual dipolar are measured. This data provides us additional long-range and local geometry restraints using a small degree of molecular alignment with the static magnetic field. We wanted to test polyethylenglycol (PEG) as a alignment medium for measuring the residual dipolar couplings (RDCs). PEG is a stable and unexpensive organic polymer that does not require a complex biochemical preparation like the most often used bacteriophage Pf1. In our study, applicability of PEG to several spin-state-selective E.COSY experiments was investigated. Measured RDCs were tested for internal consistency as refered in [2] in order to check the reliability of the values obtained in the PEG-aligned samples.

Česky

Rafinace struktury Trypanosoma U6 RNA s využitím jedno- a dvouvazebných zbytkových dipolárních interakcí.

Návaznosti

LN00A016, projekt VaV

Název: BIOMOLEKULÁRNÍ CENTRUM

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Biomolekulární centrum

Citovat

NOVÁK, Petr, Lukáš ŽÍDEK, Petr PADRTA a Vladimír SKLENÁŘ. Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1- and 2-Bond Residual Dipolar Couplings. In 19th NMR Valtice. Brno: Masarykova universita v Brně, 2004, s. 30-30. ISBN 80-210-3352-5.

@inproceedings{571832,
   author = {Novák, Petr and Žídek, Lukáš and Padrta, Petr and Sklenář, Vladimír},
   address = {Brno},
   booktitle = {19th NMR Valtice},
   keywords = {NMR; Residual dipolar couplings; RNA; structure;},
   language = {eng},
   location = {Brno},
   isbn = {80-210-3352-5},
   pages = {30-30},
   publisher = {Masarykova universita v Brně},
   title = {Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1- and 2-Bond Residual Dipolar Couplings},
   year = {2004}
}

TY  - JOUR
ID  - 571832
AU  - Novák, Petr - Žídek, Lukáš - Padrta, Petr - Sklenář, Vladimír
PY  - 2004
TI  - Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1- and 2-Bond Residual Dipolar Couplings
PB  - Masarykova universita v Brně
CY  - Brno
SN  - 8021033525
KW  - NMR
KW  - Residual dipolar couplings
KW  - RNA
KW  - structure;
N2  - The spliceosome is a complex of proteins and small nuclear RNAs (snRNAs) responsible for the removal of introns from pre-mRNA in eukaryotes. U6 snRNA has been demonstrated to be a vital element in splicing and its sequence is the most conserved of all snRNAs[1]. U6 snRNA from trypanosoma was our study. Determination of the solution structure of nucleic acid fragments is significantly improved if residual dipolar are measured. This data provides us additional long-range and local geometry restraints using a small degree of molecular alignment with the static magnetic field. We wanted to test polyethylenglycol (PEG) as a alignment medium for measuring the residual dipolar couplings (RDCs). PEG is a stable and unexpensive organic polymer that does not require a complex biochemical preparation like the most often used bacteriophage Pf1. In our study, applicability of PEG to several spin-state-selective E.COSY experiments was investigated. Measured RDCs were tested for internal consistency as refered in [2] in order to check the reliability of the values obtained in the PEG-aligned samples.
ER  -

NOVÁK, Petr, Lukáš ŽÍDEK, Petr PADRTA a Vladimír SKLENÁŘ. Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1- and 2-Bond Residual Dipolar Couplings. In \textit{19th NMR Valtice}. Brno: Masarykova universita v Brně, 2004, s.~30-30. ISBN~80-210-3352-5.

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