KLEJDUS, Bořivoj, Jitka PETRLOVÁ, David POTĚŠIL, Vojtěch ADAM, Radka MIKELOVÁ, Jan VACEK, René KIZEK and Vlastimil KUBÁŇ. Simultaneous determination of water- and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection. Analytica Chimica Acta. 2004, vol. 520, 1-2, p. 57-67. ISSN 0003-2670.
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Basic information
Original name Simultaneous determination of water- and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection.
Name in Czech Současné stanovení vitamínů rozpustných ve vodě a v tucích metodou HPLC
Authors KLEJDUS, Bořivoj (203 Czech Republic), Jitka PETRLOVÁ (203 Czech Republic), David POTĚŠIL (203 Czech Republic), Vojtěch ADAM (203 Czech Republic, guarantor), Radka MIKELOVÁ (203 Czech Republic), Jan VACEK (203 Czech Republic), René KIZEK (203 Czech Republic) and Vlastimil KUBÁŇ (203 Czech Republic).
Edition Analytica Chimica Acta, 2004, 0003-2670.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10406 Analytical chemistry
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.588
RIV identification code RIV/00216224:14310/04:00011152
Organization unit Faculty of Science
UT WoS 000223567500007
Keywords in English diode array detection; drugs; fat-soluble vitamins; high-performance liquid chromatography; mass spectrometry; pharmaceutical preparations; simultaneous determination; vitamins; water-soluble vitamins
Tags diode array detection, drugs, fat-soluble vitamins, high-performance liquid chromatography, mass spectrometry, pharmaceutical preparations, simultaneous determination, vitamins, water-soluble vitamins
Changed by Changed by: prof. RNDr. Vojtěch Adam, Ph.D., učo 63820. Changed: 22/5/2009 22:04.
Abstract
Water- and fat-soluble vitamins were separated on a MetaChem Polaris C18-A (150 mm x 4.6 mm, 3 mum particle size) in a single run using combined isocratic and linear gradient elution with a mobile phase consisting of 0.010% trifluoroacetic acid of pH 3.9 (solvent A) and methanol (solvent B) at the flow rate 0.7 ml min(-1). A linear gradient profile (A:B) started at 95:5 and was constant in the first 4 min, then linearly decreased up to 2:98 during the next 6 min, then it was constant in the next 20 min and finally linearly increased up to 95:5 ratio of water phase in the last 5 min of the separation. The most suitable detection wavelength for simultaneous vitamin determination was 280 nm. The method was applied for the solid sample of pharmaceutical preparation (B-Komplex), fortified powdered drinks (multi-vitamin) and food samples. The results were in good agreement with the declared values.
Abstract (in Czech)
Water- and fat-soluble vitamins were separated on a MetaChem Polaris C18-A (150 mm x 4.6 mm, 3 mum particle size) in a single run using combined isocratic and linear gradient elution with a mobile phase consisting of 0.010% trifluoroacetic acid of pH 3.9 (solvent A) and methanol (solvent B) at the flow rate 0.7 ml min(-1). A linear gradient profile (A:B) started at 95:5 and was constant in the first 4 min, then linearly decreased up to 2:98 during the next 6 min, then it was constant in the next 20 min and finally linearly increased up to 95:5 ratio of water phase in the last 5 min of the separation. The most suitable detection wavelength for simultaneous vitamin determination was 280 nm. The method was applied for the solid sample of pharmaceutical preparation (B-Komplex), fortified powdered drinks (multi-vitamin) and food samples. The results were in good agreement with the declared values.
Links
MSM 432100001, plan (intention)Name: Regulace biologických a technologických procesů pro konkurenceschopné zemědělství.
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