2004
Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutathione in the maize (Zea mays L.) kernels exposed to cadmium.
KLEJDUS, Bořivoj, Josef ZEHNÁLEK, Vojtěch ADAM, Jiří PETŘEK, René KIZEK et. al.Základní údaje
Originální název
Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutathione in the maize (Zea mays L.) kernels exposed to cadmium.
Název česky
HPLC/MS stanovení subpikomolárních koncentrací glutathionu v kukuřičných zrnech exponovaných kadmiu.
Autoři
KLEJDUS, Bořivoj (203 Česká republika), Josef ZEHNÁLEK (203 Česká republika), Vojtěch ADAM (203 Česká republika), Jiří PETŘEK (203 Česká republika), René KIZEK (203 Česká republika), Jan VACEK (203 Česká republika), Libuše TRNKOVÁ (203 Česká republika, garant), Roland ROZIK (703 Slovensko), Ladislav HAVEL (203 Česká republika) a Vlastimil KUBÁŇ (203 Česká republika)
Vydání
Analytica Chimica Acta, 2004, 0003-2670
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10404 Polymer science
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 2.588
Kód RIV
RIV/00216224:14310/04:00011157
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000223567500013
Klíčová slova anglicky
glutathione; HPLC/MS; voltammetry; Zea mays L.
Štítky
Změněno: 20. 6. 2008 12:09, prof. RNDr. Libuše Trnková, CSc.
V originále
Changes in fresh weight, overall protein amounts (Bradford method), cadmium concentration (DPASV) and glutathione content (HPLC/MS) were studied in maize kernels cultivated for five days at three different cadmium concentrations (0, 10 and 100 uM CdCl2). A highly sensitive HPLC/MS for determination of glutathione on a reversed-phase Atlantis dC18 chromatographic column (150 mm 2.1 mm, 3 um particle size) in an isocratic mode with acetonitrile - 0.01% TFA (5:95, flow rate 0.1 mL/min) was applied. Cadmium concentration was measured by differential pulse adsorptive stripping voltammetry (DPASV) after deposition on HMDE at potential --0.7 V (accumulation time 180 s, acetate buffer of pH 3.6) using an AUTOLAB with a VA-Stand 663 and a three-electrode system consisting of a hanging mercury drop electrode (HMDE) as a working electrode with area 0.4 mm2, an Ag/AgCl/3 M KCl as a reference electrode and a Pt-wire as an auxiliary electrode. Maize kernels exposed to the highest cadmium concentration (100 uM) germinated formerly and much better. Rapid increase of fresh weight probably relates with more intensive uptake of water in order to decrease cadmium concentration. An intensive preservation of homeostasis of Cd ions in germinating plants by defending mechanisms might explain differences of uptake rate of cadmium. The linear increase of GSH content with exposure time at all studied concentration suggests the defending mechanisms might be triggered by concentrations of a heavy metal.
Česky
Changes in fresh weight, overall protein amounts (Bradford method), cadmium concentration (DPASV) and glutathione content (HPLC/MS) were studied in maize kernels cultivated for five days at three different cadmium concentrations (0, 10 and 100 uM CdCl2). A highly sensitive HPLC/MS for determination of glutathione on a reversed-phase Atlantis dC18 chromatographic column (150 mm 2.1 mm, 3 um particle size) in an isocratic mode with acetonitrile - 0.01% TFA (5:95, flow rate 0.1 mL/min) was applied. Cadmium concentration was measured by differential pulse adsorptive stripping voltammetry (DPASV) after deposition on HMDE at potential --0.7 V (accumulation time 180 s, acetate buffer of pH 3.6) using an AUTOLAB with a VA-Stand 663 and a three-electrode system consisting of a hanging mercury drop electrode (HMDE) as a working electrode with area 0.4 mm2, an Ag/AgCl/3 M KCl as a reference electrode and a Pt-wire as an auxiliary electrode. Maize kernels exposed to the highest cadmium concentration (100 uM) germinated formerly and much better. Rapid increase of fresh weight probably relates with more intensive uptake of water in order to decrease cadmium concentration. An intensive preservation of homeostasis of Cd ions in germinating plants by defending mechanisms might explain differences of uptake rate of cadmium. The linear increase of GSH content with exposure time at all studied concentration suggests the defending mechanisms might be triggered by concentrations of a heavy metal.
Návaznosti
| LN00A081, projekt VaV |
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| MSM 432100001, záměr |
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