The fucose-binding lectin from Ralstonia solanacearum: a new
type of beta-propeller architecture formed by oligomerisation
and interacting with fucoside, fucosyllactose and plant
xyloglucan

J 2005

The fucose-binding lectin from Ralstonia solanacearum: a new type of beta-propeller architecture formed by oligomerisation and interacting with fucoside, fucosyllactose and plant xyloglucan

KOSTLÁNOVÁ, Nikola, Edward MITCHELL, Hugues LORTAT-JACOB, Stefan OSCARSON, Martina LAHMANN et. al.

Basic information

Original name

The fucose-binding lectin from Ralstonia solanacearum: a new type of beta-propeller architecture formed by oligomerisation and interacting with fucoside, fucosyllactose and plant xyloglucan

Name in Czech

Fukosu vazajici lektin z Ralstonia solanacearum: novy typ beta barelu tvoreny oligomerizaci a interakce s fukosidem, fukosyllaktosou a rostlinnym xyloglukanem

Authors

KOSTLÁNOVÁ, Nikola (203 Czech Republic), Edward MITCHELL (826 United Kingdom of Great Britain and Northern Ireland), Hugues LORTAT-JACOB (250 France), Stefan OSCARSON (752 Sweden), Martina LAHMANN (752 Sweden), Nechama GILBOA-GARBER (376 Israel), Gerard CHAMBAT (250 France), Michaela WIMMEROVÁ (203 Czech Republic, guarantor) and Anne IMBERTY (250 France)

Edition

Journal of Biological Chemistry, Bethesda, USA, Amer. Soc. Biochem. Mol. Biol. 2005, 0021-9258

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 5.854

RIV identification code

RIV/00216224:14310/05:00013768

Organization unit

Faculty of Science

UT WoS

000230678600045

Keywords in English

lectin; Ralstonia solanacearum; crystal structure; propeller; SPR

Abstract

ORIG CZ

V originále

Plant pathogens, as animal ones, use protein-carbohydrate interactions in their strategy for host recognition, attachment and invasion. The bacterium Ralstonia solanacearum which is distributed worldwide and causes lethal wilt in many agricultural crops, was shown to produce a potent L-fucose-binding lectin, RSL, a small protein of 90 amino acids with a tandem repeat in its amino acid sequence. In the present study, surface plasmon resonance experiments conducted on a series of oligosaccharides show a preference for binding to áFuc1-2Gal and áFuc1-6Gal epitopes. Titration microcalorimetry demonstrates the presence of two binding sites per monomer and an unusually high affinity of the lectin for áFuc1-2Gal containing oligosaccharides (KD 2.5 10-7 M for 2-fucosyllactose). RSL has been crystallised with a methyl derivative of fucose and with the highest affinity ligand, 2-fucosyllactose. X-ray crystal structures, the one with á-methyl-fucoside being at ultra-high resolution, reveal that each monomer consists of two small fourstranded anti-parallel â-sheets. Trimerisation through 3-fold or pseudo 3- fold axis generates a six-bladed â-propeller architecture, very similar to that previously described for the fungal lectin of Aleuria aurantia. This is the first report of a â- propeller formed by oligomerisation and not by sequential domains. Each monomer presents two fucose binding sites, resulting in six symmetrically arranged sugar binding sites for the â-propeller. Crystals were also obtained for a mutated lectin complexed with a fragment of xyloglucan, a fucosylated polysaccharides from the primary cell wall of plants, which may be the biological target of the lectin.

In Czech

Fukosu vazajici lektin z Ralstonia solanacearum: novy typ beta barelu tvoreny oligomerizaci a interakce s fukosidem, fukosyllaktosou a rostlinnym xyloglukanem

Links

LN00A016, research and development project

Name: BIOMOLEKULÁRNÍ CENTRUM

Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Center

MSM0021622413, plan (intention)

Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

Citovat

KOSTLÁNOVÁ, Nikola, Edward MITCHELL, Hugues LORTAT-JACOB, Stefan OSCARSON, Martina LAHMANN, Nechama GILBOA-GARBER, Gerard CHAMBAT, Michaela WIMMEROVÁ and Anne IMBERTY. The fucose-binding lectin from Ralstonia solanacearum: a new type of beta-propeller architecture formed by oligomerisation and interacting with fucoside, fucosyllactose and plant xyloglucan (The fucose-binding lectin from ralstonia solanacearum: a new type of beta-propeller architecture formed by oligomerisation and interacting with fucoside, fucosyllactose and plant xyloglucan). Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2005, vol. 280, No 30, p. 27839-27849. ISSN 0021-9258.

@article{571969,
   author = {Kostlánová, Nikola and Mitchell, Edward and LortatandJacob, Hugues and Oscarson, Stefan and Lahmann, Martina and GilboaandGarber, Nechama and Chambat, Gerard and Wimmerová, Michaela and Imberty, Anne},
   article_location = {Bethesda, USA},
   article_number = {30},
   keywords = {lectin; Ralstonia solanacearum; crystal structure; propeller; SPR},
   language = {eng},
   issn = {0021-9258},
   journal = {Journal of Biological Chemistry},
   title = {The fucose-binding lectin from Ralstonia solanacearum: a new type of beta-propeller architecture formed by oligomerisation and interacting with fucoside, fucosyllactose and plant xyloglucan},
   volume = {280},
   year = {2005}
}

TY  - JOUR
ID  - 571969
AU  - Kostlánová, Nikola - Mitchell, Edward - Lortat-Jacob, Hugues - Oscarson, Stefan - Lahmann, Martina - Gilboa-Garber, Nechama - Chambat, Gerard - Wimmerová, Michaela - Imberty, Anne
PY  - 2005
TI  - The fucose-binding lectin from Ralstonia solanacearum: a new type of beta-propeller architecture formed by oligomerisation and interacting with fucoside, fucosyllactose and plant xyloglucan
JF  - Journal of Biological Chemistry
VL  - 280
IS  - 30
SP  - 27839-27849
EP  - 27839-27849
PB  - Amer. Soc. Biochem. Mol. Biol.
SN  - 00219258
KW  - lectin
KW  - Ralstonia solanacearum
KW  - crystal structure
KW  - propeller
KW  - SPR
N2  - Plant pathogens, as animal ones, use protein-carbohydrate interactions in their strategy for host recognition, attachment and invasion. The bacterium Ralstonia solanacearum which is distributed worldwide and causes lethal wilt in many agricultural crops, was shown to produce a potent L-fucose-binding lectin, RSL, a small protein of 90 amino acids with a tandem repeat in its amino acid sequence. In the present study, surface plasmon resonance experiments conducted on a series of oligosaccharides show a preference for binding to áFuc1-2Gal and áFuc1-6Gal epitopes. Titration microcalorimetry demonstrates the presence of two binding sites per monomer and an unusually high affinity of the lectin for áFuc1-2Gal containing oligosaccharides (KD 2.5 10-7 M for 2-fucosyllactose). RSL has been crystallised with a methyl derivative of fucose and with the highest affinity ligand, 2-fucosyllactose. X-ray crystal structures, the one with á-methyl-fucoside being at ultra-high resolution, reveal that each monomer consists of two small fourstranded anti-parallel â-sheets. Trimerisation through 3-fold or pseudo 3- fold axis generates a six-bladed â-propeller architecture, very similar to that previously described for the fungal lectin of Aleuria aurantia. This is the first report of a â- propeller formed by oligomerisation and not by sequential domains. Each monomer presents two fucose binding sites, resulting in six symmetrically arranged sugar binding sites for the â-propeller. Crystals were also obtained for a mutated lectin complexed with a fragment of xyloglucan, a fucosylated polysaccharides from the primary cell wall of plants, which may be the biological target of the lectin.
ER  -

KOSTLÁNOVÁ, Nikola, Edward MITCHELL, Hugues LORTAT-JACOB, Stefan OSCARSON, Martina LAHMANN, Nechama GILBOA-GARBER, Gerard CHAMBAT, Michaela WIMMEROVÁ and Anne IMBERTY. The fucose-binding lectin from Ralstonia solanacearum: a new type of beta-propeller architecture formed by oligomerisation and interacting with fucoside, fucosyllactose and plant xyloglucan (The fucose-binding lectin from ralstonia solanacearum: a new type of beta-propeller architecture formed by oligomerisation and interacting with fucoside, fucosyllactose and plant xyloglucan). \textit{Journal of Biological Chemistry}. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2005, vol.~280, No~30, p.~27839-27849. ISSN~0021-9258.

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