Characterization of presumptive Staphylococcus xylosus strains
by SDS-PAGE protein profiles.

D 2005

Characterization of presumptive Staphylococcus xylosus strains by SDS-PAGE protein profiles.

NOVÁKOVÁ, Dana, Vlastimil ŠTĚTINA, Ivo SEDLÁČEK a Petr PETRÁŠ

Základní údaje

Originální název

Characterization of presumptive Staphylococcus xylosus strains by SDS-PAGE protein profiles.

Název česky

Charakterizace presumptivních kmenů Staphylococcus xylosus pomocí SDS-PAGE proteinových profilů.

Autoři

NOVÁKOVÁ, Dana (203 Česká republika, garant), Vlastimil ŠTĚTINA (203 Česká republika), Ivo SEDLÁČEK (203 Česká republika) a Petr PETRÁŠ (203 Česká republika)

Vydání

Sborník. 1. vydání. Brno, IX. Pracovní setkání biochemiků a molekulárních biologů. Sborník příspěvků. 27 s. 2005

Nakladatel

Masarykova universita v Brně

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/05:00012492

Organizační jednotka

Přírodovědecká fakulta

ISBN

80-210-3635-4

Klíčová slova anglicky

Staphylococcus xylosus; Staphylococcus equorum; SDS-PAGE; clinical specimen

Štítky

clinical specimen, SDS-PAGE, Staphylococcus equorum, Staphylococcus xylosus

Změněno: 3. 2. 2010 15:01, prof. RNDr. Ivo Sedláček, CSc.

Anotace

ORIG CZ

V originále

Staphylococcus xylosus and Staphylococcus equorum are two biochemically and phylogenetically close species. Athough S.xylosus is usually only transient on human and is primarily acquired from domestic animal and their products, it has been isolated also from human clinical specimens, mainly from urinary tract infection. Lack of the biochemical key tests makes differentiating of these species difficult. It is very important to find another reliable approach to distinguish S.xylosus and S.equorum species. A group of 18 presumptive S.xylosus strains from human clinical isolates were analysed by biochemical tests and ribotyping. SDS-PAGE protein analysis was performed to confirm the results of ribotyping. Biochemical properties were tested by API Staph and ID 32 STAPH commercial kits as well as by conventional tests. Ribotyping was done with EcoRI and HindIII restriction enzymes and a probe complementary to 16S-23S rRNA. Electrophoresis of proteins was performed using a 5% polyacrylamide stacking gel and 12% separated gel containing 0.1% SDS. Biochemical tests identified 17 S.xylosus strains and one intermediated S.xylosus/S.equorum strain. Ribotyping in contrast to biotyping recognised 4 strains of S.equorum and one strain that shows high similarity to Staphylococcus nepalensis reference strains. Protein analysis divided tested strains into four groups. A group with four S.equorum strains, a second group which probably contains one S.nepalensis strain. Our data indicate that SDS-PAGE as well as ribotyping have got a higher discriminative power than biotyping to distinguish S.xylosus and S.equorum strains and that are able to identified them.

Česky

Skupina 18 presumptivních kmenů Staphylococcus xylosus pocházejících z humánního klinického materiálu byla charakterizováno za použití biotypizace, ribotypizace a analýzy celobuněčných proteinů metodou SDS-PAGE. Analýzou proteinů a také ribotypizací byly kmeny identifikovány jako zástupci druhu S.xylosus, 4 kmeny náležely do taxonu S. equorum a jeden kmen vykazuje podobnost k S. nepalensis referenčním kmenům.

Návaznosti

GA301/02/1505, projekt VaV

Název: Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků

Investor: Grantová agentura ČR, Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků

Citovat

NOVÁKOVÁ, Dana, Vlastimil ŠTĚTINA, Ivo SEDLÁČEK a Petr PETRÁŠ. Characterization of presumptive Staphylococcus xylosus strains by SDS-PAGE protein profiles. In IX. Pracovní setkání biochemiků a molekulárních biologů. Sborník příspěvků. Sborník. 1. vydání. Brno: Masarykova universita v Brně, 2005, 27 s. ISBN 80-210-3635-4.

@inproceedings{572115,
   author = {Nováková, Dana and Štětina, Vlastimil and Sedláček, Ivo and Petráš, Petr},
   address = {Brno},
   booktitle = {IX. Pracovní setkání biochemiků a molekulárních biologů. Sborník příspěvků.},
   edition = {Sborník. 1. vydání},
   keywords = {Staphylococcus xylosus; Staphylococcus equorum; SDS-PAGE; clinical specimen},
   language = {eng},
   location = {Brno},
   isbn = {80-210-3635-4},
   publisher = {Masarykova universita v Brně},
   title = {Characterization of presumptive Staphylococcus xylosus strains by SDS-PAGE protein profiles.},
   year = {2005}
}

TY  - JOUR
ID  - 572115
AU  - Nováková, Dana - Štětina, Vlastimil - Sedláček, Ivo - Petráš, Petr
PY  - 2005
TI  - Characterization of presumptive Staphylococcus xylosus strains by SDS-PAGE protein profiles.
PB  - Masarykova universita v Brně
CY  - Brno
SN  - 8021036354
KW  - Staphylococcus xylosus
KW  - Staphylococcus equorum
KW  - SDS-PAGE
KW  - clinical specimen
N2  - Staphylococcus xylosus and Staphylococcus equorum are two biochemically and phylogenetically close species. Athough S.xylosus is usually only transient on human and is primarily acquired from domestic animal and their products, it has been isolated also from human clinical specimens, mainly from urinary tract infection. Lack of the biochemical key tests makes differentiating of these species difficult. It is very important to find another reliable approach to distinguish S.xylosus and S.equorum species. A group of 18 presumptive S.xylosus strains from human clinical isolates were analysed by biochemical tests and ribotyping. SDS-PAGE protein analysis was performed to confirm the results of ribotyping. Biochemical properties were tested by API Staph and ID 32 STAPH commercial kits as well as by conventional tests. Ribotyping was done with EcoRI and HindIII restriction enzymes and a probe complementary to 16S-23S rRNA. Electrophoresis of proteins was performed using a 5% polyacrylamide stacking gel and 12% separated gel containing 0.1% SDS. Biochemical tests identified 17 S.xylosus strains and one intermediated S.xylosus/S.equorum strain. Ribotyping in contrast to biotyping recognised 4 strains of S.equorum and one strain that shows high similarity to Staphylococcus nepalensis reference strains. Protein analysis divided tested strains into four groups. A group with four S.equorum strains, a second group which probably contains one S.nepalensis strain. Our data indicate that SDS-PAGE as well as ribotyping have got a higher discriminative power than biotyping to distinguish S.xylosus and S.equorum strains and that are able to identified them.
ER  -

NOVÁKOVÁ, Dana, Vlastimil ŠTĚTINA, Ivo SEDLÁČEK a Petr PETRÁŠ. Characterization of presumptive Staphylococcus xylosus strains by SDS-PAGE protein profiles. In \textit{IX. Pracovní setkání biochemiků a molekulárních biologů. Sborník příspěvků.}. Sborník. 1. vydání. Brno: Masarykova universita v Brně, 2005, 27 s. ISBN~80-210-3635-4.

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