Ribotyping of sucrose - negative mesophilic Aeromonas sp.

SEDLÁČEK, Ivo and Dana NOVÁKOVÁ. Ribotyping of sucrose - negative mesophilic Aeromonas sp. In Abstract book. 2005.

Basic information

• Original name: Ribotyping of sucrose - negative mesophilic Aeromonas sp.
• Name in Czech: Ribotypizace mezofilních sacharózo negativních Aeromonas sp.
• Authors: SEDLÁČEK, Ivo (203 Czech Republic, guarantor) and Dana NOVÁKOVÁ (203 Czech Republic).
• Edition: Abstract book, 2005.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Canada
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/05:00013913
• Organization unit: Faculty of Science
• Keywords in English: Aeromonas; ribotyping; identification
• Tags: Aeromonas, identification, ribotyping

Abstract

Aeromonads are ubiquitous waterborne organisms isolated from both environmental sources and clinical material. During the past few years the taxonomy of the genus Aeromonas has been changed and several new species were proposed. Methods used to type aeromonads are based either on phenotypic characteristics or on the analysis of their genomes. In total 11 sucrose-negative isolates of aeromonads were obtained from fresh water in 2004. Biochemical identification by commercial kit ENTEROtest24 (Pliva-Lachema) was supplemented with additional tube and plate tests and results were evaluated by software TNW (Pliva-Lachema). Ribotyping of selected isolates and reference cultures using EcoRI and HindIII restriction enzymes and DNA probe complementary to 16S and 23S rRNA was accomplished. Majority species of the genus Aeromonas produce acid from sucrose except A. jandaei, A. schubertii, A. trota or A. eucrenophila species. Biochemical identification to the species level of isolates with negative acid production from sucrose was unsuccessful. Biotyping confirmed that all 11 isolates belong to the genus Aeromonas. Ribotyping profiles from EcoRI and HindIII of analysed isolates gave unique band pattern with no relationship to the selected reference aeromonas cultures. Five sucrose-negative isolates with positive acid production from inositol demonstrated good correlation among results of phenotyping and ribotyping in this study and differed from the remaining six isolates which shoved heterogeneous hybridization profiles. Ribotyping appeared to be a good tool for the differentiation of aeromonads and mentioned five isolates with unique biotype probably represent a novel species.

Abstract (in Czech)

Aeromonády jsou především vodní organismy, ale vyskytují se také v prostředí i v klinickém materiálu. Během několika posledních let došlo v taxonomii aeromonád ke změnám a k popisu nových druhů. Metody použité k typizaci aeromonád jsou založeny jak na fenotypové charakteristice, tak i na analýze jejich genomu. Celkem 11 sacharózo negativních izolátů aeromonád bylo izolováno ze sladkovodního prostředí v roce 2004. Biochemcká identifikace byla provedena komerční soupravou ENTEROtest24 (Pliva-Lachema) a identifikace programem TNW (Pliva-Lachema), robotypizace vybraných izolátů byla pomocí restrikčních enzymů EcoRI a HindIII a sondou komplementární k 16S a 23S rRNA. Biotypizace potvrdila, že všech 11 izolátů patří k rodu Aeromonas, avšak jejich hybridizační profil byl naprosto jedinečný bez návaznosti na ribotypy referenčních kultur. Pět sacharózo negativních izolátů s pozitivní tvorbou kyseliny z inositolu pravděpodobně reprezentuje nový druh aeromonády.

Links

• MSM0021622416, plan (intention): Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time