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@inproceedings{577001,
author = {Křížová, Jana and Španová, Alena and Rittich, Bohuslav},
address = {Ljubljana, Slovenia},
booktitle = {The 19th international ICFMH symposium FoodMicro 2004: New tools for improving microbial food safety and quality. Biotechnology and molecular biology approaches. 12-16th September 2004, Portorož, Slovenia},
keywords = {Bifidobacterium spp.; identification; genus and species specific PCR; ARDRA},
language = {eng},
location = {Ljubljana, Slovenia},
isbn = {961-90346-3-5},
pages = {151-151},
publisher = {Slovenian Microbiology Society},
title = {Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis},
year = {2004}
}
TY - JOUR
ID - 577001
AU - Křížová, Jana - Španová, Alena - Rittich, Bohuslav
PY - 2004
TI - Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis
PB - Slovenian Microbiology Society
CY - Ljubljana, Slovenia
SN - 9619034635
KW - Bifidobacterium spp.
KW - identification
KW - genus and species specific PCR
KW - ARDRA
N2 - Molecular methods were applied to verify the species identification of Bifidobacterium isolates from the Culture Collection of Dairy Microorganisms (CCDM, Tábor, ČR), previously identified by fenotypic techniques only. The methods used for identification in this study were the genus and species specific PCR and restriction analysis of amplified ribosomal DNA restriction analysis with three restriction enzymes. The methods were introduced and optimalised using type and reference strains of Bifidobacterium species. The molecular methods chosen enabled species identification of the analysed strains and provided congruent results.
ER -
KŘÍŽOVÁ, Jana, Alena ŠPANOVÁ a Bohuslav RITTICH. Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis. In \textit{The 19th international ICFMH symposium FoodMicro 2004: New tools for improving microbial food safety and quality. Biotechnology and molecular biology approaches. 12-16th September 2004, Portorož, Slovenia}. Ljubljana, Slovenia: Slovenian Microbiology Society, 2004, s.~151. ISBN~961-90346-3-5.
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